Verticillium wilt (VW) is caused by Verticillium dahliae and significantly impacts cotton yield and quality. Breeding of resistant varieties is the most effective strategy for controlling VW. Therefore, it is important to identify VW resistance genes. The diploid wild cotton species Gossypium australe is highly resistant to VW. This study identified 33 CC-NBS-LRR (CNL) genes in the G. australe genome. GauCNL expression pattern (EP) analyses showed that EP1 cluster which included four of the 33 genes, were significantly up-regulated after V. dahliae inoculation. The EP1 cluster genes were then introduced into Arabidopsis thaliana wild type Col-0, respectively. The results showed GauCNL18 overexpression significantly increased V. dahliae resistance, indicating the functional importance of GauCNL18. GauCNL18 promotes V. dahliae resistance by activating the salicylic acid signaling pathway to mediate up-regulating pathogenesis-related genes and reactive oxygen species accumulation. This study identified a CNL resistance gene, GauCNL18, and revealed its resistance mechanism to V. dahliae.

黄萎病（VW）是由大丽轮枝菌引起的，严重影响棉花产量和质量。培育抗病品种是控制大众的最有效策略。因此，识别大众汽车抗性基因很重要。二倍体野生棉种 Gossypium australe对大众有很强的抵抗力。该研究在G. australe基因组中鉴定了 33 个 CC-NBS-LRR (CNL) 基因。GauCNL表达模式 (EP) 分析表明，包含 33 个基因中的四个的 EP1 簇在接种大丽花后显着上调。然后将EP1簇基因分别引入拟南芥野生型Col-0。结果显示GauCNL18过表达显着增加了大丽花抗性，表明GauCNL18的功能重要性。GauCNL18通过激活水杨酸信号通路介导上调发病相关基因和活性氧的积累来促进大丽花抗性。本研究鉴定了一个CNL抗性基因GauCNL18，并揭示了其对大丽花弧菌的抗性机制。