A fluorescent probe (Probe 1) for the visible colorimetric detection of tyrosinase (Tyr) was developed. The quantitative range of detection of Tyr activity detected was 0 to 800 u/mL with the detection limit (LOD) was 5.00 u/mL. The probe solution emitted increasing yellow-green luminescence with increasing Tyr activity, which was observed under a 365 nm UV lamp. In addition, the green (G) and red (R) values of these photos had a linear relationship with the activity of Tyr. Probe 1 could be used for the quantitative and quantification detection of Tyr activity in real fruits samples using a smartphone. Colorimetry with catechol as the substrate was used to determine the accuracy of this method. This work shows that Probe 1 can be used for the quantitative detection of Tyr using just a smartphone.

中文翻译：

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一种用于酪氨酸酶可见光比色检测的荧光探针

开发了一种用于酪氨酸酶 (Tyr) 可见光比色检测的荧光探针（探针 1）。Tyr 活性的检测定量范围为 0 至 800 u/mL，检测限 (LOD) 为 5.00 u/mL。在 365 nm 紫外灯下观察到，随着 Tyr 活性的增加，探针溶液发出越来越多的黄绿色发光。此外，这些照片的绿色（G）和红色（R）值与Tyr的活性呈线性关系。探针 1 可用于使用智能手机对真实水果样品中的 Tyr 活性进行定量和定量检测。以邻苯二酚为底物的比色法用于确定该方法的准确性。这项工作表明 Probe 1 可用于仅使用智能手机对 Tyr 进行定量检测。