To better understand the high recalcitrance of bamboo during bioconversion, the fine spatial distribution of polymers in bamboo was studied with Imaging FTIR microscopy under both transmission and ATR modes, combined with PCA data processing. The results demonstrated that lignin, xylan and hydroxycinnamic acid (HCA) were more concentrated in the fibers near the xylem conduit, while cellulose was evenly distributed across the whole fiber sheath. PCA processing produced a clear separation between bamboo fibers and parenchyma cells, indicating that the parenchyma cells contains more pectin and HCA than fibers. It also demonstrated that cellulose, xylan and S-lignin were concentrated most heavily in bamboo fiber secondary cell walls, while G-lignin, pectin and HCA were found more in the compound middle lamella. The revealed information regarding polymer distribution is of great significance for better understanding of the inherent design mechanism of plant cell wall and its efficient utilization.

为了更好地了解竹子在生物转化过程中的高度顽固性，在透射和 ATR 模式下，结合 PCA 数据处理，使用成像 FTIR 显微镜研究了竹子中聚合物的精细空间分布。结果表明，木质素、木聚糖和羟基肉桂酸 (HCA) 更集中在木质部导管附近的纤维中，而纤维素均匀分布在整个纤维鞘中。PCA 处理在竹纤维和薄壁细胞之间产生了明显的分离，表明薄壁细胞含有比纤维更多的果胶和 HCA。还表明纤维素、木聚糖和S-木质素在竹纤维次生细胞壁中含量最高，而G-木质素、果胶和HCA在复合中层中含量较多。