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Optometry for a short-sighted microscope
Biophysical Journal ( IF 3.4 ) Pub Date : 2021-09-10 , DOI: 10.1016/j.bpj.2021.09.011
Carine Julien 1 , Martin Oheim 2
Affiliation  

Evanescent-wave scattering is a topic in classical electrodynamics and in the study of colloidal particles near a boundary. However, how such near-surface scattering at subcellular refractive-index heterogeneities degrades the excitation confinement in biological total internal reflection fluorescence microscopy has not been well studied. An elegant theoretical work by Axelrod and Axelrod now addresses this very relevant question and reveals that—even when scattered—evanescent light preserves some of its surprising optical properties.



中文翻译:

近视显微镜的验光

倏逝波散射是经典电动力学和边界附近胶体粒子研究中的一个主题。然而,这种亚细胞折射率异质性下的近表面散射如何降低生物全内反射荧光显微镜中的激发限制尚未得到很好的研究。Axelrod 和 Axelrod 的一项优雅的理论工作现在解决了这个非常相关的问题,并揭示了即使在散射的情况下,倏逝光也保留了其一些令人惊讶的光学特性。

更新日期:2021-10-19
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