A novel electrochemical glassy carbon electrode modified with a multi-walled carbon nanotube, cytochrome c (Cyt c) and zinc oxide nanoparticles (ZnONPs) was fabricated to increase the sensitivity of electrode for the detection of streptomycin (STN) in certain pharmaceutical samples. Cyclic voltammetry (CV) and differential pulse voltammetry techniques were used for an electrochemical characterization of the electrode. Furthermore, the electrochemical biosensor construction phases were examined by using X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR). Under the optimal experimental conditions, the electrode offers a high selectivity and sensitivity signaling in the co-existence method of STN with the linear concentration ranging from 0.02 to 2.2 μM. The detection limits (LOD) and limit of quantification (LOQ) were found to be 0.0028 and 0.0562 μM, respectively. The fabricated sensing electrode has good stability, reproducibility and sensitivity towards STN in the pharmaceutical samples. Preliminary determinations of binding sites within the specified grid box size, which covers both Cyt c and STN, were done by molecular docking analysis. Moreover, density functional theory (DFT) computations were performed to provide insightful information into the optimized geometry of STN.

一种用多壁碳纳米管、细胞色素c (Cyt c) 和氧化锌纳米颗粒 (ZnONPs) 被制造以提高电极检测某些药物样品中链霉素 (STN) 的灵敏度。循环伏安法 (CV) 和差分脉冲伏安法技术用于电极的电化学表征。此外，通过使用 X 射线衍射 (XRD)、透射电子显微镜 (TEM) 和傅里叶变换红外光谱 (FTIR) 检查电化学生物传感器的构建阶段。在最佳实验条件下，该电极在 STN 共存方法中提供了高选择性和灵敏度的信号，线性浓度范围为 0.02 至 2.2 μM。发现检测限 (LOD) 和定量限 (LOQ) 分别为 0.0028 和 0.0562 μM。制备的传感电极对药物样品中的STN具有良好的稳定性、重现性和灵敏度。在指定的网格框大小内初步确定结合位点，它涵盖了 Cytc和 STN，通过分子对接分析完成。此外，还进行了密度泛函理论 (DFT) 计算，以提供有关 STN 优化几何结构的深入信息。