Hydrogen peroxide (H₂O₂) is a main reactive oxygen by-product produced in the metabolism of organisms and a common biomarker of oxidative stress. Aggregation-induced emission (AIE) probes for H₂O₂ have been proposed. Such AIEgens mostly use benzeneboronic acid as a recognition group. Recently, a strategy involving enzyme-catalyzed polymerization of AIE compounds shows great potential in AIEgens design. We herein modify the AIE motif, tetraphenylethene (TPE) with o-phenylenediamine (TPE-TAF), which can be oxidated by H₂O₂ in HRP to form an intramolecular phenazine structure. Compared with a similar approach, the proposed strategy is simple and the TPE-TAF showed a sensitive “turn-on” fluorescence with H₂O₂. The detection limit (LOD) is 3.39 μM and the probe is highly specific against H₂O₂. We further verified the reaction mechanism of the enzyme-catalyzed coupling reaction. The probe is a promising candidate as a stable and safe fluorescent substrate in H₂O₂ sensing.

过氧化氢 (H ₂ O ₂ ) 是生物体代谢中产生的主要活性氧副产物，也是氧化应激的常见生物标志物。已经提出了H ₂ O _{2 的}聚集诱导发射(AIE)探针。此类AIEgens大多使用苯硼酸作为识别基团。最近，涉及 AIE 化合物的酶催化聚合的策略在 AIEgens 设计中显示出巨大的潜力。我们在这里用邻苯二胺 (TPE-TAF)修饰了 AIE 基序四苯乙烯(TPE)，它可以被 H ₂ O ₂氧化在 HRP 中形成分子内吩嗪结构。与类似的方法相比，所提出的策略很简单，TPE-TAF 显示出与 H ₂ O ₂敏感的“开启”荧光。检测限 (LOD) 为 3.39 μM，探针对 H ₂ O ₂具有高度特异性。我们进一步验证了酶催化偶联反应的反应机理。该探针是 H ₂ O ₂传感中稳定且安全的荧光底物的有前途的候选者。