Nitric oxide (NO) is a highly reactive gas molecule, exhibiting antimicrobial properties. Because of its reactive nature, it is challenging to store and deliver NO efficiently as a therapeutic agent. The objective of this study was to develop NO-releasing polymeric fibers (NO-fibers), as an effective delivery platform for NO. NO-fibers were fabricated with biopolymer solutions of polyvinyl pyrrolidone (PVP) and ethylcellulose (EC), and derivatives of N-diazeniumdiolate (NONOate) as NO donor molecules, using an electrospinning system. We evaluated in vitro NO release kinetics, along with antimicrobial effects and cytotoxicity in microorganisms and human cell culture models. We also studied the long-term stability of NONOates in NO-fibers over 12 months. We demonstrated that the NO-fibers could release NO over 24 h, and showed inhibition of the growth of Pseudomonas aeruginosa (P. aeruginosa) and methicillin-resistant Staphylococcus aureus (MRSA), without causing cytotoxicity in human cells. NO-fibers were able to store NONOates for over 12 months at room temperature. This study presents the development of NO-fibers, and the feasibility of NO-fibers to efficiently store and deliver NO, which can be further developed as a bandage.

中文翻译：

---

抗菌一氧化氮释放纤维的研制

一氧化氮 (NO) 是一种高活性气体分子，具有抗菌特性。由于其反应性质，将 NO 作为治疗剂有效地储存和输送具有挑战性。本研究的目的是开发释放 NO 的聚合物纤维 (NO-纤维)，作为 NO 的有效输送平台。NO 纤维是用聚乙烯吡咯烷酮 (PVP) 和乙基纤维素 (EC) 的生物聚合物溶液以及N 的衍生物制成的-diazeniumdiolate (NONOate) 作为 NO 供体分子，使用静电纺丝系统。我们评估了体外 NO 释放动力学，以及微生物和人类细胞培养模型中的抗菌作用和细胞毒性。我们还研究了 NONOates 在 12 个月内在 NO 纤维中的长期稳定性。我们证明 NO 纤维可以在 24 小时内释放 NO，并显示出对铜绿假单胞菌 (P. aeruginosa) 和耐甲氧西林金黄色葡萄球菌 (MRSA) 的生长有抑制作用，而不会对人体细胞造成细胞毒性。NO 纤维能够在室温下储存 NONOates 超过 12 个月。本研究介绍了 NO 纤维的发展，以及 NO 纤维有效储存和输送 NO 的可行性，可进一步开发为绷带。