In this work, a surface plasmon resonance (SPR) based immunosensor was prepared by the immobilization of the amine-functionalized gold nanoparticles (N-AuNPs) on the sensing surface to sense immunoglobulin M (IgM) antibodies in the aqueous solution and artificial plasma. The characterization studies of SPR based immunosensor for IgM detection were performed with scanning electron microscope (SEM), contact angle measurements, and ellipsometry. Kinetic studies for the IgM immunosensor were carried out in the range of 1.0 to 200 ng/mL IgM concentrations in an aqueous solution. The total IgM analysis time including adsorption, desorption, and regeneration cycles was nearly 10 min for the prepared immunosensor. The limit of detection (LOD) and limit of quantification (LOQ) were found as 0.08 and 0.26 ng/mL, respectively. The reusability of the proposed immunosensor was tested with 6 consecutive adsorption-desorption, and regeneration cycles. Also, enzyme-linked immunosorbent assay (ELISA) method was utilized in the validation of the immunosensor.

中文翻译：

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用于金纳米粒子 Igm 检测的基于表面等离子体共振的免疫传感器

在这项工作中，通过将胺功能化的金纳米粒子 (N-AuNPs) 固定在传感表面上以检测水溶液和人工血浆中的免疫球蛋白 M (IgM) 抗体，制备了基于表面等离子体共振 (SPR) 的免疫传感器。用于 IgM 检测的基于 SPR 的免疫传感器的表征研究是通过扫描电子显微镜 (SEM)、接触角测量和椭圆光度法进行的。IgM 免疫传感器的动力学研究在 1.0 到 200 ng/mL IgM 的水溶液浓度范围内进行。对于制备的免疫传感器，包括吸附、解吸和再生循环在内的总 IgM 分析时间接近 10 分钟。检测限 (LOD) 和定量限 (LOQ) 分别为 0.08 和 0.26 ng/mL。所提出的免疫传感器的可重复使用性通过 6 个连续的吸附-解吸和再生循环进行了测试。此外，酶联免疫吸附测定（ELISA）方法被用于免疫传感器的验证。