Abstract

This review considers the methods of probe, electron, and confocal microscopy for studying the structure of the yeast Candida. The latest advances in the study of antimicrobial effects by high-resolution microscopy are presented. The advantages and disadvantages of each method are presented, as well as the limits of their application. Processes such as cell wall lysis and destruction of the cytoplasmic membrane caused by antifungal drugs are described, as well as their effect on the nanostructural or mechanical properties of Candida. Particular attention is paid to sample preparation of Candida and scanning protocols.

中文翻译：

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纳米技术在念珠菌酵母结构研究中的应用

摘要

本综述考虑了用于研究酵母念珠菌结构的探针、电子和共聚焦显微镜方法。介绍了高分辨率显微镜抗菌作用研究的最新进展。介绍了每种方法的优点和缺点，以及它们的应用限制。描述了由抗真菌药物引起的细胞壁裂解和细胞质膜破坏等过程，以及它们对念珠菌的纳米结构或机械性能的影响。特别注意念珠菌的样品制备和扫描协议。