Abstract—

An analysis of the acute and subacute toxicity of a copper oxide nanocomposite (NC) encapsulated in natural polymeric matrix of arabinogalactan is presented. The study was carried out in two stages. At the first stage, the acute toxicity of the NC was studied. For this, white mice were orally administered one time with an aqueous solution of NC at a dose 2000 mg/kg. The study demonstrated that according to this parameter, NC belongs to low-toxicity substances of hazard class 5. At the second stage, the biological response of the white rats was evaluated with a subacute intragastric effect of Cu₂ОAG NC at a dose of 500 µg/kg (based on Cu) body weight within 10 days. The study demonstrated an increase in the contents of copper in the brain tissue, liver, and kidneys compared with the control group. Morphological and neurophysiological changes were detected in white rats treated with this NC. A pronounced macrophage reaction consisting in an increase in the number of Kupffer cells in the organ sinusoids was observed in liver tissue. In the tissues of the sensorimotor cortex of the brain, a sharp decrease in the number of astroglia cells was detected, which indicates a selective cytotoxicity of this NC. When studying the bioelectric activity of the sensorimotor cortex of the brain in animals a decrease in β-2 rhythm of background electroencephalogram, as well as a decrease in the amplitude of the P2 and N2-P3 peaks of the visually evoked potentials, were observed. No genotoxic effects were detected under the influence of the NC.

中文翻译：

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包裹在阿拉伯半乳聚糖聚合物基质中的 Cu2O 纳米复合材料的毒性分析

摘要——

对封装在阿拉伯半乳聚糖天然聚合物基质中的氧化铜纳米复合材料 (NC) 的急性和亚急性毒性进行了分析。该研究分两个阶段进行。在第一阶段，研究了NC的急性毒性。为此，白色小鼠以 2000 mg/kg 的剂量口服 NC 水溶液一次。研究表明，根据该参数，NC属于危害等级5的低毒物质。 第二阶段，以Cu ₂的亚急性胃内作用评价白鼠的生物学反应ОAG NC 在 10 天内以 500 µg/kg（基于铜）体重的剂量给药。研究表明，与对照组相比，脑组织、肝脏和肾脏中的铜含量有所增加。在用这种 NC 治疗的白色大鼠中检测到形态学和神经生理学变化。在肝组织中观察到明显的巨噬细胞反应，包括器官血窦中库普弗细胞数量的增加。在大脑感觉运动皮层的组织中，检测到星形胶质细胞数量急剧减少，这表明该 NC 具有选择性细胞毒性。在研究动物大脑感觉运动皮层的生物电活动时，背景脑电图的 β-2 节律降低，以及观察到视觉诱发电位的 P2 和 N2-P3 峰的幅度减小。在 NC 的影响下未检测到基因毒性作用。