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Rapid conversion of IgG to biosensor using an antibody-binding protein-based probe
Analyst ( IF 3.6 ) Pub Date : 2021-09-03 , DOI: 10.1039/d1an01171e Guangwei Zhao 1 , Haimei Li 1 , Baowei Li 1 , Mei Li 1 , Wei Xu 1 , Limei Chen 1 , Baoqiang Wang 2 , Jinhua Dong 1, 3
Analyst ( IF 3.6 ) Pub Date : 2021-09-03 , DOI: 10.1039/d1an01171e Guangwei Zhao 1 , Haimei Li 1 , Baowei Li 1 , Mei Li 1 , Wei Xu 1 , Limei Chen 1 , Baoqiang Wang 2 , Jinhua Dong 1, 3
Affiliation
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We successfully developed a fluorescent probe that can quickly convert full-length antibodies to Quenchbodies, which represent a type of fluorescent immunosensor with high binding affinity and specificity depending on the reaction of antigens and antibodies. An anti-testosterone IgG was successfully converted to an immunosensor that detects testosterone with a limit of detection (LOD) of 0.76 nM and concentration for 50% of maximal effect (EC50) of 61.5 nM. Another IgG-based immunosensor detected ractopamine with an LOD of 15.5 pM and EC50 of 48.6 nM.
中文翻译：
使用基于抗体结合蛋白的探针将 IgG 快速转化为生物传感器
我们成功开发了一种荧光探针，可以将全长抗体快速转化为淬灭体，这是一种依赖于抗原和抗体反应的具有高结合亲和力和特异性的荧光免疫传感器。抗睾酮 IgG 成功转化为免疫传感器，检测睾酮的检测限 (LOD) 为 0.76 nM，50% 的最大效应浓度 (EC 50 ) 为 61.5 nM。另一种基于 IgG 的免疫传感器检测到莱克多巴胺，LOD 为 15.5 pM，EC 50为 48.6 nM。
更新日期：2021-09-10
中文翻译：
使用基于抗体结合蛋白的探针将 IgG 快速转化为生物传感器
我们成功开发了一种荧光探针，可以将全长抗体快速转化为淬灭体，这是一种依赖于抗原和抗体反应的具有高结合亲和力和特异性的荧光免疫传感器。抗睾酮 IgG 成功转化为免疫传感器，检测睾酮的检测限 (LOD) 为 0.76 nM，50% 的最大效应浓度 (EC 50 ) 为 61.5 nM。另一种基于 IgG 的免疫传感器检测到莱克多巴胺，LOD 为 15.5 pM，EC 50为 48.6 nM。
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