Enhancing the specific surface area of stationary phase is important in chromatographic science, especially in open-tubular column in which the coating only exists on the inner surface. In this work, a porous layer open-tubular (PLOT) column with stationary phase of styrene and itaconic acid-copolymerized polymer was developed. Thermal-initiated polymerization method with strategies like controlling the ratio of reaction reagents to solvents and reaction time, confinement by the narrow inner diameter of capillary were used for preparing the stationary phase with uniform structure and relatively thick layer. Due to the high separation efficiency and capacity, the PLOT column was used for capillary electrochromatography (CEC) separation of multiple groups of analytes like alkylbenzenes, phenyl amines, phenols, vanillins, and sulfonamides with theoretical plates (N) up to 1,54,845 N/m. In addition, due to high permeability of the CEC column and large electroosmotic flow mobility generated by abundant carboxyl groups in the coating material, the PLOT-CEC column was successfully coupled with mass spectrometry (MS) through a sheath flow interface. The developed PLOT-CEC-MS method was used for the analysis of antiseptics like parabens and herbicides like pyridines.

中文翻译：

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用于毛细管电色谱-质谱分析的以苯乙烯和衣康酸共聚聚合物为固定相的多孔层开管柱

提高固定相的比表面积在色谱科学中非常重要，特别是在涂层仅存在于内表面的开管柱中。在这项工作中，开发了一种具有苯乙烯和衣康酸共聚聚合物固定相的多孔层开管 (PLOT) 柱。采用热引发聚合法,通过控制反应试剂与溶剂的比例、反应时间、毛细管窄内径限制等策略制备结构均匀、层厚较厚的固定相。由于高分离效率和容量，PLOT 色谱柱用于毛细管电色谱 (CEC) 分离多组分析物，如烷基苯、苯胺、苯酚、香草醛、和磺胺类药物，理论塔板数 (N) 高达 1,54,845 N/m。此外，由于 CEC 柱的高渗透性和涂层材料中丰富的羧基产生的大电渗流动迁移率，PLOT-CEC 柱通过鞘流界面成功地与质谱 (MS) 耦合。开发的 PLOT-CEC-MS 方法用于分析防腐剂（如对羟基苯甲酸酯）和除草剂（如吡啶）。