Neuronal calcium sensor 1 (NCS1) dependent modulation of neuronal morphology and development,The FASEB Journal

当前位置： X-MOL 学术 › FASEB J. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Neuronal calcium sensor 1 (NCS1) dependent modulation of neuronal morphology and development
The FASEB Journal ( IF 4.4 ) Pub Date : 2021-09-09 , DOI: 10.1096/fj.202100731r
Tom T Fischer _{1,

2} , Lien D Nguyen _{1,

3} , Barbara E Ehrlich _{1,

3,

4}

Affiliation

Calcium (Ca²⁺) signaling is critical for neuronal functioning and requires the concerted interplay of numerous Ca²⁺-binding proteins, including neuronal calcium sensor 1 (NCS1). Although an important role of NCS1 in neuronal processes and in neurodevelopmental and neurodegenerative diseases has been established, the underlying mechanisms remain enigmatic. Here, we systematically investigated the functions of NCS1 in the brain. Using Golgi-Cox staining, we observed a reduction in dendritic complexity and spine density in the prefrontal cortex and the dorsal hippocampus of Ncs1^−/− mice, which may underlie concomitantly observed deficits in memory acquisition. Subsequent RNA sequencing of Ncs1^−/− and Ncs1^+/+ mouse brain tissues revealed that NCS1 modulates gene expression related to neuronal morphology and development. Investigation of developmental databases further supported a molecular role of NCS1 during brain development by identifying temporal gene expression patterns. Collectively, this study provides insights into NCS1-dependent signaling and lays the foundation for a better understanding of NCS1-associated diseases.

中文翻译：

神经元钙传感器 1 (NCS1) 对神经元形态和发育的依赖性调节

钙 (Ca ²⁺ ) 信号传导对神经元功能至关重要，需要众多 Ca ²⁺结合蛋白的协同相互作用，包括神经元钙传感器 1 (NCS1)。虽然 NCS1 在神经元过程以及神经发育和神经退行性疾病中的重要作用已经确立，但其潜在机制仍然是个谜。在这里，我们系统地研究了 NCS1 在大脑中的功能。使用 Golgi-Cox 染色，我们观察到Ncs1 ^-/-小鼠前额叶皮层和背侧海马的树突复杂性和脊柱密度降低，这可能是同时观察到的记忆获取缺陷的基础。Ncs1的后续 RNA 测序^-/-和Ncs1 ^+/+小鼠脑组织显示，NCS1 调节与神经元形态和发育相关的基因表达。通过识别时间基因表达模式，对发育数据库的研究进一步支持了 NCS1 在大脑发育过程中的分子作用。总的来说，这项研究提供了对 NCS1 依赖性信号传导的见解，并为更好地了解 NCS1 相关疾病奠定了基础。

更新日期：2021-09-10

点击分享查看原文

点击收藏

阅读更多本刊最新论文