Cyanobacterial biomass is a rich renewable resource for direct bioelectricity generation in biomass-based microbial fuel cells (MFCs). In this study, the performance of a double chamber MFC with Anabaena vaginicola cyanobacterial biomass as the anodic substrate was investigated. The anode chamber was inoculated with a pure culture of Shewanella oneidensis MR-1, and different MFC experiments using pretreated and untreated cyanobacterial biomass substrate with 5 or 20 mg/L riboflavin were conducted. Pretreatment of the biomass and addition of 20 mg/L riboflavin led to the highest current density (366 mA/m³), maximum power density (144 mW/m³), chemical oxygen demand removal efficiency (65%), and coulombic efficiency (5.7%). Increasing the concentration of riboflavin improved bioelectricity generation (1.4–1.7-fold increase in maximum power density), confirming its effectiveness as a redox mediator, and its role in the extracellular electron transfer process of the S. oneidensis MR-1 biofilm. Moreover, pretreating the biomass also enhanced the conversion efficiency of biomass to bioelectricity. This indicated that pretreatment of the biomass influenced the utilization of biomass by S. oneidensis MR-1 cells. These findings can help increasing the potential of using renewable substrates for more efficient bioenergy generation in pure culture MFCs.

蓝藻生物质是一种丰富的可再生资源，可在基于生物质的微生物燃料电池 (MFC) 中直接生物发电。在这项研究中，研究了以阴道鱼腥藻蓝藻生物质为阳极底物的双室 MFC 的性能。阳极室接种了Shewanella oneidensis MR-1的纯培养物，并使用含有 5 或 20 mg/L 核黄素的预处理和未处理的蓝藻生物质基质进行了不同的 MFC 实验。生物质预处理和添加 20 mg/L 核黄素导致最高电流密度 (366 mA/m ³ )、最大功率密度 (144 mW/m ³)、化学需氧量去除效率 (65%) 和库仑效率 (5.7%)。增加核黄素的浓度可改善生物发电（最大功率密度增加 1.4-1.7 倍），证实其作为氧化还原介质的有效性，以及其在S. oneidensis MR-1 生物膜的细胞外电子转移过程中的作用。此外，对生物质进行预处理还提高了生物质向生物电的转化效率。这表明生物质的预处理影响了S. oneidensis MR-1 细胞对生物质的利用。这些发现有助于提高使用可再生基质在纯培养 MFC 中更有效地产生生物能源的潜力。