It has previously been shown that preeclampsia is associated with disturbed hemostasis and that extracellular vesicles (EVs) play important role in the regulation of hemostatic homeostasis. Thus, we hypothesized that the altered procoagulant characteristics of circulating platelet-derived EVs may contribute to the disturbed hemostasis in preeclampsia.

Using multicolor flow cytometry, we have analyzed both tissue factor expressing procoagulant EVs and platelet-derived EV subpopulations derived from resting and activated thrombocytes by examining them in plasma samples of preeclamptic patients and pregnancy-matched healthy individuals.

Compared to pregnancy-matched healthy individuals in preeclamptic patients a significantly (p < 0.05) higher ratio of Annexin-V positive activated platelets and a higher number of CD142⁺ tissue factor bearing procoagulant EVs were found, whereas the absolute amount of circulating CD41a⁺ platelet-derived EVs and CD62P⁺/CD41a⁺ EVs produced by activated thrombocytes was significantly lower in the plasma of preeclamptic women. In the plasma samples, there was no significant difference in the amount of CD63⁺ platelet-derived EVs.

We propose that increased platelet activation and tissue factor expression of platelet derived extracellular vesicles may contribute to the hypercoagulable state observed in preeclampsia.

先前已经表明，先兆子痫与止血障碍有关，并且细胞外囊泡 (EV) 在止血稳态的调节中起重要作用。因此，我们假设循环血小板衍生 EV 的促凝特性改变可能导致先兆子痫的止血障碍。

使用多色流式细胞仪，我们通过在先兆子痫患者和妊娠匹配的健康个体的血浆样本中检查它们，分析了表达促凝 EV 和源自静息和活化血小板的血小板衍生 EV 亚群的组织因子。

在先兆子痫患者中，与妊娠匹配的健康个体相比，Annexin-V 阳性活化血小板的比例显着 (p < 0.05) 更高，携带 CD142 ^{+组织因子的促凝 EV 的数量也更高，而循环 CD41a +}血小板的绝对量先兆子痫妇女血浆中由活化血小板产生的衍生 EV 和 CD62P ⁺ /CD41a ⁺ EV 显着降低。^{在血浆样本中，CD63 +}血小板衍生 EV的数量没有显着差异。

我们提出，血小板衍生的细胞外囊泡的血小板活化和组织因子表达增加可能导致先兆子痫中观察到的高凝状态。