Accurate determination of antibiotic resistance genes (ARGs) in environmental DNA molecules (eDNA) is challenging owing to its low abundance in the aquatic environment. Here we report a facile and cost-efficient approach to extract trace amount of eDNAs in the aquatic environment using LnPO₄ nanomaterials. Among the nanomaterials, less crystalline TbPO₄ nanoneedles was identified as the most prominent candidate for long stranded DNA and short stranded DNA with adsorption efficiency above 97%. The adsorbed DNA was washed off from TbPO₄ nanoneedles by optimized eluant (85% PBS, 15% EtOH, 4 g/L glycine, pH 10.0) with an optimal DNA recovery of 78.83%. Our approach showed a comparable or better eDNA extraction efficiency than a commercial extraction method for different environmental samples, but 89% less cost. The high purity of the extracted eDNA was demonstrated by a high A_260/280 ratio. Using qPCR experiment, the occurrence of six common ARGs in the eDNA were detected with abundance ranging from 4.06 × 10³ to 3.51 × 10⁹ copies/L in river samples. This specific DNA capturer is valuable for the evaluation of spatial and temporal dynamic of ARGs pollution to provide insight into the potential risk with regard to the human health.

由于其在水生环境中的丰度低，准确测定环境 DNA 分子 (eDNA) 中的抗生素抗性基因 (ARG) 具有挑战性。在这里，我们报告了一种使用 LnPO ₄纳米材料在水生环境中提取痕量 eDNA 的简便且经济高效的方法。在纳米材料中，结晶度较低的 TbPO ₄纳米针被确定为长链 DNA 和短链 DNA 的最突出候选者，吸附效率高于 97%。_{从 TbPO 4}洗掉吸附的 DNA通过优化的洗脱液（85% PBS、15% EtOH、4 g/L 甘氨酸、pH 10.0）制备纳米针，最佳 DNA 回收率为 78.83%。对于不同的环境样品，我们的方法显示出与商业提取方法相当或更好的 eDNA 提取效率，但成本降低了 89%。_{高 A 260/280}比率证明了提取的 eDNA 的高纯度。使用qPCR实验，在河流样品中检测到eDNA中存在6种常见ARG，丰度范围为4.06 × 10 ³到3.51 × 10 ⁹拷贝/L。这种特定的 DNA 捕获器对于评估 ARGs 污染的空间和时间动态很有价值，可以深入了解与人类健康有关的潜在风险。