Hydrogen peroxide (H₂O₂) widely involves in intracellular and intercellular redox signaling pathways, playing a vital role in regulating various physiological events. Nevertheless, current analytical methods for the H₂O₂ assay are often hindered by relatively long response time, low sensitivity, or self-interference. Herein, a zeolitic imidazolate framework-8 (ZIF-8)-based surface-enhanced Raman scattering (SERS) sensor has been developed to detect H₂O₂ released from living cells by depositing ZIF-8 over SERS active gold nanoparticles (AuNPs) grafted with H₂O₂-responsive probe molecules, 2-mercaptohydroquinone. Combining the superior fingerprint identification of SERS and the highly efficient enrichment and selective response of H₂O₂ by ZIF, the ZIF-8-based SERS sensor exhibits a high anti-interference ability for H₂O₂ detection, with a limit of detection as low as 0.357 nM. Satisfyingly, owing to the enhanced catalytic activity derived from the successful integration of AuNPs and ZIF, the response time as short as 1 min can be obtained, demonstrating the effectiveness of the SERS sensor for rapid H₂O₂ detection. Furthermore, the developed SERS sensor enables real-time detection of H₂O₂ secreted from living cells under phorbol myristate acetate stimulation, as cells can be cultured on-chip. This study will pave the way toward the development of a metal–organic framework-based SERS platform for application in the fields of biosensing and early disease diagnosis associated with H₂O₂ secretion, thus exhibiting promising potential for future therapies.

中文翻译：

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使用基于 ZIF-8 的表面增强拉曼散射传感器原位监测活细胞释放的过氧化氢

过氧化氢（H ₂ O ₂）广泛参与细胞内和细胞间氧化还原信号通路，在调节各种生理事件中起着至关重要的作用。然而，目前用于 H ₂ O ₂测定的分析方法通常受到相对较长的响应时间、低灵敏度或自干扰的阻碍。在此，开发了一种基于沸石咪唑酯骨架-8 (ZIF-8) 的表面增强拉曼散射 (SERS) 传感器，通过在 SERS 活性金纳米粒子 (AuNP) 上沉积 ZIF-8来检测活细胞释放的H ₂ O ₂ H ₂ O ₂接枝-响应探针分子，2-巯基氢醌。结合SERS优越的指纹识别和ZIF对H ₂ O ₂的高效富集和选择性响应，基于ZIF-8的SERS传感器对H ₂ O ₂检测表现出很高的抗干扰能力，具有检测限低至 0.357 nM。令人满意的是，由于 AuNPs 和 ZIF 的成功整合提高了催化活性，可以获得短至 1 分钟的响应时间，证明了 SERS 传感器在快速检测H ₂ O _{2 方面}的有效性。此外，开发的 SERS 传感器能够实时检测 H ₂ O ₂在佛波醇肉豆蔻酸酯醋酸盐刺激下从活细胞分泌，因为细胞可以在芯片上培养。该研究将为开发基于金属-有机框架的 SERS 平台铺平道路，该平台可用于生物传感和与 H ₂ O ₂分泌相关的早期疾病诊断领域，从而为未来的治疗展现出广阔的潜力。