Glioblastoma (GBM) is one of the most common and malignant types of primary cancer in the central nervous system; however, the clinical outcomes of patients with GBM remain poor. Circular RNAs (circRNAs) have been revealed to serve important roles in diverse biological processes, such as regulating cell proliferation, epithelial‑mesenchymal transition and tumor development. However, the underlying biological function of circRNA filamin A (circFLNA) and its potential role in GBM remain to be determined. The present study aimed to identify differentially expressed circRNAs in GBM. Reverse transcription‑quantitative PCR was used to analyze the expression levels of circFLNA. The results demonstrated that the expression levels of circFLNA were significantly upregulated in clinical GBM samples and GBM cells compared with adjacent healthy brain tissues and normal human astrocytes, respectively. The results of the Cell Counting Kit‑8 and Transwell assays revealed that circFLNA knockdown significantly inhibited the proliferative and invasive abilities of GBM cell lines. Moreover, high circFLNA expression levels were associated with a worse prognosis in GBM. MicroRNA (miR)‑199‑3p was subsequently predicted to be target of circFLNA. The inhibitory effect of miR‑199‑3p on cell proliferation and invasion was partially reversed following circFLNA knockdown. In conclusion, the findings of the present study identified novel roles for circFLNA in GBM and indicated that the circFLNA/miR‑199‑3p signaling axis may serve an important role in GBM progression. Therefore, circFLNA may represent a novel target for the diagnosis and treatment of GBM.

中文翻译：

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circFLNA 通过负调控 miR-199-3p 的表达促进胶质母细胞瘤的增殖和侵袭。

胶质母细胞瘤 (GBM) 是中枢神经系统中最常见和恶性的原发性癌症之一。然而，GBM 患者的临床结果仍然很差。已发现环状 RNA (circRNA) 在多种生物过程中发挥重要作用，例如调节细胞增殖、上皮间质转化和肿瘤发展。然而，circRNA细丝蛋白A（circFLNA）的潜在生物学功能及其在GBM中的潜在作用仍有待确定。本研究旨在鉴定 GBM 中差异表达的 circRNA。逆转录定量 PCR 用于分析 circFLNA 的表达水平。结果表明，与邻近的健康脑组织和正常人星形胶质细胞相比，临床GBM样本和GBM细胞中circFLNA的表达水平显着上调。Cell Counting Kit-8 和 Transwell 检测的结果表明，circFLNA 敲低显着抑制了 GBM 细胞系的增殖和侵袭能力。此外，高 circFLNA 表达水平与 GBM 预后较差有关。MicroRNA (miR)‑199‑3p 随后被预测为 circFLNA 的靶标。在 circFLNA 敲低后，miR-199-3p 对细胞增殖和侵袭的抑制作用被部分逆转。综上所述，本研究的结果确定了 circFLNA 在 GBM 中的新作用，并表明 circFLNA/miR-199-3p 信号轴可能在 GBM 进展中发挥重要作用。因此，circFLNA可能代表了GBM诊断和治疗的新靶点。