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Nanopore sequencing of tomato mottle leaf distortion virus, a new bipartite begomovirus infecting tomato in Brazil
Archives of Virology ( IF 2.7 ) Pub Date : 2021-09-08 , DOI: 10.1007/s00705-021-05220-w
Thais P Martins 1, 2 , Tadeu A Souza 2 , Patricia S da Silva 2 , Erich Y T Nakasu 2 , Fernando L Melo 1 , Alice K Inoue-Nagata 2 , Tatsuya Nagata 1
Affiliation  

During a survey in a tomato field in Luziânia (Goiás State, Brazil), a single plant with mottling, chlorotic spots, and leaf distortion was found. A new bipartite begomovirus sequence was identified using nanopore sequence technology and confirmed by Sanger sequencing. The highest nucleotide sequence identity match of the DNA-A component (2596 bases) was 81.64% with tomato golden leaf deformation virus (HM357456). Due to the current species demarcation criterion of 91% nucleotide sequence identity for DNA-A, we propose this virus to be a new member of the genus Begomovirus, named "tomato mottle leaf distortion virus".



中文翻译:

番茄斑驳叶变形病毒的纳米孔测序,一种感染巴西番茄的新型二分贝戈莫病毒

在 Luziânia(巴西戈亚斯州)的番茄田中进行的一项调查中,发现了一株带有斑驳、褪绿斑点和叶片变形的单株植物。使用纳米孔序列技术鉴定了一个新的二分贝戈莫病毒序列,并通过 Sanger 测序进行了确认。DNA-A组分(2596个碱基)与番茄金叶变形病毒(HM357456)的最高核苷酸序列同一性匹配率为81.64%。由于目前DNA-A 91% 核苷酸序列同一性的物种划分标准,我们建议该病毒为Begomovirus属的新成员,命名为“番茄斑驳叶畸变病毒”。

更新日期:2021-10-07
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