Plant SNF1-Related Kinase1 (SnRK1) is an evolutionarily conserved energy-sensing protein kinase that orchestrates transcriptional networks to maintain cellular energy homeostasis when energy supplies become limited. However, the mechanism by which SnRK1 regulates this gene expression switch to gauge cellular energy status remains largely unclear. In this work, we show that the rice histone H3K27me3 demethylase JMJ705 is required for low energy stress tolerance in rice plants. The genetic inactivation of JMJ705 resulted in similar effects as those of the rice snrk1 mutant on the transcriptome, which impairs not only the promotion of the low energy stress-triggered transcriptional program but also the repression of the program under an energy-sufficient state. We show that the α-subunit of OsSnRK1 interacts with and phosphorylates JMJ705 to stimulate its H3K27me3 demethylase activity. Further analysis revealed that JMJ705 directly targets a set of low energy stress-responsive transcription factor genes. These results uncover the chromatin mechanism of SnRK1-regulated gene expression in both energy-sufficient and -limited states in plants and suggest that JMJ705 functions as an upstream regulator of the SnRK1α-controlled transcriptional network.

中文翻译：

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SnRK1 刺激组蛋白 H3K27me3 去甲基化酶 JMJ705 调节转录开关以控制能量稳态


植物 SNF1 相关激酶 1 (SnRK1) 是一种进化上保守的能量感应蛋白激酶，可在能量供应有限时协调转录网络以维持细胞能量稳态。然而，SnRK1 调节这种基因表达开关以测量细胞能量状态的机制仍不清楚。在这项工作中，我们证明水稻组蛋白 H3K27me3 去甲基酶 JMJ705 是水稻植物低能量胁迫耐受性所必需的。 JMJ705的基因失活导致了与水稻snrk1突变体相似的转录组效应，不仅损害了低能胁迫触发的转录程序的促进，而且损害了该程序在能量充足状态下的抑制。我们发现 OsSnRK1 的 α 亚基与 JMJ705 相互作用并磷酸化 JMJ705，以刺激其 H3K27me3 去甲基酶活性。进一步分析表明，JMJ705直接针对一组低能量应激反应转录因子基因。这些结果揭示了植物中能量充足和有限状态下 SnRK1 调节基因表达的染色质机制，并表明 JMJ705 作为 SnRK1α 控制转录网络的上游调节因子。