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An α-D-galactan and a β-D-glucan from the mushroom Amanita muscaria: Structural characterization and antitumor activity against melanoma
Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2021-09-08 , DOI: 10.1016/j.carbpol.2021.118647
Matheus Zavadinack 1 , Daniel de Lima Bellan 2 , Jessica Loren da Rocha Bertage 1 , Shayane da Silva Milhorini 1 , Edvaldo da Silva Trindade 2 , Fernanda Fogagnoli Simas 2 , Guilherme Lanzi Sassaki 1 , Lucimara M C Cordeiro 1 , Marcello Iacomini 1
Affiliation  

Polysaccharides α-D-galactan (GAL-Am) and β-D-glucan (GLC-Am) were obtained from Amanita muscaria fruiting bodies. They were purified using different methodologies, such as Fehling precipitation (for both fractions), freeze-thawing process and ultrafiltration (for GLC-Am). Results showed that the GAL-Am has (1 → 6)-linked Galp main chain branched at O-2 by terminal Galp units and has not been previously reported. Besides, GLC-Am has (1 → 3)-linked Glcp in the main chain, substituted at O-6 by (1 → 6)-linked β-Glcp units. Both are water-soluble, with 9.0 × 103 g/moL and 1.3 × 105 g/moL, respectively. GAL-Am and GLC-Am presented a selective proliferation reduction against B16-F10 melanoma cell line, not affecting non tumoral BALB/3T3 fibroblast cell line. Furthermore, both fractions reduced clonogenic capacity of melanoma cell line over an extended period of time. These results were obtained without modulations in B16-F10 cell adhesion, reinforcing the biological activities towards cell proliferation impairment and eliciting these polysaccharides as promising compounds to further exploration of their antimelanoma properties.



中文翻译:

来自蘑菇毒蝇伞的 α-D-半乳聚糖和 β-D-葡聚糖:结构表征和抗黑色素瘤的抗肿瘤活性

多糖α-D-半乳聚糖(GAL-Am)和β-D-葡聚糖(GLC-Am)从毒蝇伞子实体中获得。使用不同的方法对它们进行纯化,例如 Fehling 沉淀(用于两种馏分)、冻融工艺和超滤(用于 GLC-Am)。结果表明,GAL-Am 具有 (1 → 6)-连接的 Gal p主链,其通过末端 Gal p单元在O -2处分支,并且以前没有报道过。此外,GLC-Am在主链上具有(1→3)-连接的Glc p ,在O -6 处被(1→6)-连接的β-Glc p单元取代。两者都是水溶性的,分别为 9.0 × 10 3  g/moL 和 1.3 × 10 5 克/摩尔,分别。GAL-Am 和 GLC-Am 对 B16-F10 黑色素瘤细胞系有选择性的增殖抑制作用,不影响非肿瘤性 BALB/3T3 成纤维细胞系。此外,两种组分在延长的时间段内都降低了黑色素瘤细胞系的克隆形成能力。这些结果是在没有调节 B16-F10 细胞粘附的情况下获得的,增强了对细胞增殖损伤的生物活性,并引发了这些多糖作为有前景的化合物,以进一步探索它们的抗黑色素瘤特性。

更新日期:2021-09-20
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