Systematic electron ionization fragmentation studies of steroids have been performed to elucidate and trace their characteristic fragmentation patterns. However, the electron ionization source setting at 70 eV electron energy is much higher than the ionization potential (7–15 eV) of most organic compounds, leading to extensive fragmentation. We present a multifactorial study on optimizing a low-energy electron ionization source to maximize molecular ion formation while minimizing the extent of fragmentation to improve the analytical sensitivity of steroids, especially the more thermolabile ones, while preserving the information that can be extracted from the data.

中文翻译：

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使用高分辨率质谱法进行类固醇组学分析的低能电子电离优化

已经对类固醇进行了系统的电子电离碎裂研究，以阐明和追踪它们的特征碎裂模式。然而，设置在 70 eV 电子能量的电子电离源远高于大多数有机化合物的电离电位 (7-15 eV)，从而导致广泛的碎裂。我们提出了一项关于优化低能电子电离源的多因素研究，以最大限度地增加分子离子的形成，同时最大限度地减少碎裂程度，以提高类固醇的分析灵敏度，尤其是更不耐热的类固醇，同时保留可以从数据中提取的信息.