Recent methodological advances have opened new perspectives for tropical dendrochonological studies by facilitating the visualization, delimitation, and analyses of tree-rings. One of those improvements was brought by X-ray densitometry, which allows building radial wood density profiles at microscopic scale. Furthermore, recent methods allow for cutting long histological sections to study anatomical variations along the entire radius of trees. These techniques have mainly been applied to low wood-density species from temperate and Mediterranean regions, with only limited applications in the tropics. Here we provide an improved protocol that allows for obtaining long histological sections of tropical woods, apply it to six species with varying wood densities 0.45−0.85 g cm⁻³ (Eucalyptus grandis, Tectona grandis, Acacia mangium, Cedrela fissilis, Hymenaea courbaril, and Copaifera duckei), and explore potential applications for tree-ring analyses. We provide instructions on core-microtome knife adjustments, procedures for softening and sectioning long histological samples of high wood-density species. We also present a multi-proxy approach that combines X-ray density profiles with the histological sections that improve the characterization and distinction of the various and complex tropical growth rings anatomical markers (fibre zone, marginal parenchyma, and ring porosity). This multi-proxy approach also opens the door for obtaining quantitative anatomy and physical parameters of tropical species with (intra-annual resolution. Our proposed approach is thus not only an additional tool to improve ring-boundary delimitation of tropical species, but it also paves the way to more innovative, borderline approaches in tropical dendrochronology.

最近的方法学进步通过促进树木年轮的可视化、定界和分析，为热带树栖动物学研究开辟了新的视角。其中一项改进是 X 射线密度测定法带来的，它允许在微观尺度上构建径向木材密度分布。此外，最近的方法允许切割长的组织切片来研究沿树木整个半径的解剖变化。这些技术主要应用于温带和地中海地区的低木材密度树种，仅在热带地区应用有限。在这里，我们提供了一种改进的协议，允许获得热带木材的长组织学切片，将其应用于具有不同木材密度 0.45−0.85 g cm ^-3 ( Eucalyptus grandis, Tectona grandis , Acacia mangium , Cedrela fissilis , Hymenaea courbaril和Copaifera dadei)，并探索树轮分析的潜在应用。我们提供有关核心切片机刀调整、软化和切片高木材密度物种的长组织学样本的程序的说明。我们还提出了一种多代理方法，该方法将 X 射线密度剖面与组织切片相结合，以改善各种复杂热带年轮解剖标记（纤维区、边缘薄壁组织和环孔隙率）的表征和区分。这种多代理方法还为获得热带物种的定量解剖和物理参数打开了大门（年内分辨率。因此，我们提出的方法不仅是改善热带物种环边界划定的附加工具，而且还为更创新的方式，