Liquid chromatography (LC) and supercritical fluid chromatography (SFC) are powerful separation techniques for forensic science in the analysis of drugs, toxicology samples, trace evidence, and explosives. Combining LC and SFC with diode array detectors (DAD) can provide detection at a single wavelength or generate complete UV spectra. This advantageous pairing within forensic science allows distinguishing between classes and subclasses of compounds, discriminating positional isomers with benzene substitutions, and using library database comparison to enhance certainty of compound identification through statistical comparison. Additionally, employing DAD at single wavelengths and generation of UV spectra allow quantitation with excellent linearity, good linear range, and satisfactory limits of detection. Important for quantitation, the generation of UV spectra allows confirmation of compound identity and peak purity. The purpose of this article is to provide an overview of the qualitative and quantitative uses of LC, and SFC paired with scanning DAD within forensic science over the last ten years (2010–2020), including the analysis of seized drugs, toxicology samples, explosives, inks, and dyes. Potential benefits, limitations, and future directions for liquid phase separation paired with ultraviolet detection are considered.

液相色谱 (LC) 和超临界流体色谱 (SFC) 是法医学分析药物、毒理学样品、痕量证据和爆炸物的强大分离技术。将 LC 和 SFC 与二极管阵列检测器 (DAD) 相结合，可以提供单一波长的检测或生成完整的紫外光谱。法医学中的这种有利配对允许区分化合物的类别和亚类，区分具有苯取代的位置异构体，并使用库数据库比较通过统计比较来增强化合物识别的确定性。此外，在单一波长下使用 DAD 并生成 UV 光谱允许以优异的线性、良好的线性范围和令人满意的检测限进行定量。对于定量很重要，紫外光谱的产生可以确认化合物的身份和峰纯度。本文的目的是概述过去十年（2010-2020 年）法医科学中 LC 和 SFC 与扫描 DAD 配对的定性和定量用途，包括分析缉获的药物、毒理学样品、爆炸物、油墨和染料。考虑了液相分离与紫外检测相结合的潜在好处、局限性和未来方向。