The mesoporous supports of alkylated poly(ionic liquid)s (PILs) are got through free radical copolymerization. With an immobilization yield of 99% for Candida antarctica lipase B (CalB), the biocatalyst showed high advantage for biodiesel production under mild conditions. Typical catalyst CalB_0.2@PIL-C8 was capable of effectively converting soybean oil into biodiesel with a yield of 68.2%, which is higher than Novozym 435 for 57.5%. The biodiesel yield was almost 1.5 folds compared to that of free CalB under the same conditions. And the catalyst remained about 80% activity in a seven-run recovery test with no CalB leaching in the whole process. The hydrophobic PILs not only provide a suitable reaction micro-environment but also can help CalB keep an open conformation in immobilizing with its flexible hydrophobic alkyl chains. This research offered a green and effective strategy for regulating the hydrophobicity and surface potential of support to enhance the performance of immobilized lipase.

烷基化聚（离子液体）（PIL）的介孔载体是通过自由基共聚得到的。南极念珠菌脂肪酶 B (CalB)的固定化率为 99% ，该生物催化剂在温和条件下显示出生物柴油生产的高度优势。典型催化剂 CalB _0.2@PIL-C8 能够有效地将豆油转化为生物柴油，产率为 68.2%，高于 Novozym 435 的 57.5%。在相同条件下，生物柴油的产量几乎是游离 CalB 的 1.5 倍。并且在整个过程中没有 CalB 浸出的情况下，催化剂在七次运行的回收率测试中保持了大约 80% 的活性。疏水性 PILs 不仅提供了合适的反应微环境，而且还可以帮助 CalB 保持开放构象以固定其灵活的疏水性烷基链。该研究为调节载体的疏水性和表面电位以提高固定化脂肪酶的性能提供了一种绿色有效的策略。