Dynamic Excimer (DYNEX) Imaging of Lipid Droplets,ACS Sensors

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Dynamic Excimer (DYNEX) Imaging of Lipid Droplets
ACS Sensors ( IF 8.2 ) Pub Date : 2021-09-09 , DOI: 10.1021/acssensors.1c01206
M Carmen Gonzalez-Garcia ₁ , Carmen Salto-Giron ₁ , Pilar Herrero-Foncubierta _{1,

2} , Tomás Peña-Ruiz ₃ , Maria Dolores Giron-Gonzalez ₄ , Rafael Salto-Gonzalez ₄ , Angel Perez-Lara _{1,

5} , Amparo Navarro ₃ , Emilio Garcia-Fernandez ₁ , Angel Orte ₁

Affiliation

Unraveling cellular physiological processes via luminescent probes that target specific cellular microenvironments is quite challenging due to the uneven distribution of probes. Herein, we designed a new dynamic excimer (DYNEX) imaging method that involves the sensitive detection of nanosecond-scale dynamic molecular contacts of a fluorescent acridone derivative and reveals the cell microenvironment polarity. Using our method, we specifically tracked cell lipid droplets in fibroblast colon carcinoma cells. These organelles play a central role in metabolic pathways, acting as energy reservoirs in regulatory processes. DYNEX imaging provides the inner polarity of cell lipid droplets, which can be related to lipid contents and metabolic dysfunctions. This new methodology will inspire development of novel multidimensional fluorescent sensors that are able to provide target-specific and orthogonal information at the nanosecond scale.

中文翻译：

脂质液滴的动态准分子 (DYNEX) 成像

由于探针分布不均，通过针对特定细胞微环境的发光探针来解开细胞生理过程非常具有挑战性。在此，我们设计了一种新的动态准分子 (DYNEX) 成像方法，该方法涉及对荧光吖啶酮衍生物的纳秒级动态分子接触的灵敏检测，并揭示细胞微环境的极性。使用我们的方法，我们专门跟踪了成纤维细胞结肠癌细胞中的细胞脂滴。这些细胞器在代谢途径中起着核心作用，在调节过程中充当能量库。DYNEX 成像提供细胞脂滴的内部极性，这可能与脂质含量和代谢功能障碍有关。

更新日期：2021-10-22

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