Abstract

Five new glycosides including mimenghuasu A and B (1-2), isolinarin (3), cyclocitralosides A and B (4–5), along with forty-seven known compounds were isolated from the flower buds of Buddleja officinalis. These structures were elucidated by extensive spectroscopic analysis (UV, IR, 1 D, 2 D NMR, and MS spectra). The anti-inflammatory activities of the isolated compounds were determined by enzyme-linked immunosorbent assay (ELISA) on the expression of TNF-α (LPS-activated RAW264.7 cells) and MTT experiment on LPS-induced HUVECs proliferation effects. Good suppressive effects on the expression of TNF-α were shown by 4 and 5 with IC₅₀ values of 19.35 and 22.10 μM, respectively, compared to positive control indomethacin (IC₅₀ 16.40 μM). In addition to this, some isolated compounds exhibited excellent antioxidant activities including compounds 16, 18, 29, 39, and 47 (IC₅₀ μM: 82.59, 72.94, 33.65, 46.67, and 20.81, respectively) with almost the same or stronger potency with reference to vitamin C as positive control (IC₅₀ 81.83 μM).

摘要

5 种新的糖苷包括迷梦花素 A 和 B ( 1 - 2 )、异亚麻苷 ( 3 )、环柠檬苷 A 和 B ( 4-5 )，以及 47 种已知化合物是从Buddleja officinalis的花蕾中分离出来的。通过广泛的光谱分析（UV、IR、1D、2D NMR 和 MS 光谱）阐明了这些结构。通过酶联免疫吸附试验（ELISA）对TNF - α（LPS激活的RAW264.7细胞）的表达和MTT实验对LPS诱导的HUVECs增殖作用测定分离化合物的抗炎活性。对 TNF -α表达的良好抑制作用由4和5与阳性对照消炎痛 (IC ₅₀ 16.40 μM)相比，IC _{50值分别为 19.35 和 22.10 μM。}除此之外，一些分离的化合物表现出优异的抗氧化活性，包括化合物16、18、29、39和47 （ IC 50 _μM：分别为 82.59、72.94、33.65、46.67 和 20.81），具有几乎相同或更强的效力参考维生素 C 作为阳性对照 (IC ₅₀ 81.83 μM)。