Abstract

A new muurolane-type sesquiterpene, a new flavone arabinofuranoside derivative, and other five known flavone arabinofuranoside derivatives were isolated from the leaves of Fissistigma bicolor (Annonaceae family). Their chemical structures were determined to be (1S,6R,7S)-muurola-4,10(14)-diene-15-ol (1), quercetin 3-O-β-D-apiofuranosyl-(1→2)-α-L-arabinofuranoside (2), quercetin 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinofuranoside (3), quercetin 3-O-α-L-arabinofuranoside (4), kaempferol 3-O-β-D-apiofuranosyl-(1→2)-α-L-arabinofuranoside (5), kaempferol 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinofuranoside (6), and kaempferol 3-O-α-L-arabinofuranoside (7) by analyses of HR-ESI-MS and NMR spectral data. Compounds 4 and 7 containing monosaccharide, arabinofuranoside, potentially inhibited NO productions in LPS activated RAW264.7 cells (IC₅₀ 13.4 ± 0.5 and 12.6 ± 0.4 µM) in compared to disaccharide derivatives (IC₅₀ ranging from 58.9 ± 3.3 to 65.6 ± 3.8 µM).

摘要

从Fissistigma bicolor（番荔枝科）的叶子中分离出一种新的 muurolane 型倍半萜烯、一种新的黄酮阿拉伯呋喃糖苷衍生物和其他五种已知的黄酮阿拉伯糖呋喃糖苷衍生物。它们的化学结构被确定为(1 S ,6 R ,7 S )-muurola-4,10(14)-diene-15-ol ( 1 ), quercetin 3- O - β -D-apiofuranosyl-(1→ 2) -α -L-阿拉伯呋喃糖苷( 2 )、槲皮素3 - O - α -L-吡喃鼠李糖基-(1→2) -α -L-阿拉伯呋喃糖苷( 3 )、槲皮素3 - O - α-L-阿拉伯呋喃糖苷( 4 )、山柰酚3- O - β -D-呋喃糖基-(1→2)- α -L-阿拉伯呋喃糖苷( 5 )、山柰酚3- O - α -L-吡喃鼠李糖基-(1→2) - α -L-阿拉伯呋喃糖苷 ( 6 ) 和山奈酚 3- O - α -L-阿拉伯呋喃糖苷 ( 7 )，通过 HR-ESI-MS 和 NMR 光谱数据分析。含有单糖阿拉伯呋喃糖苷的化合物4和7可能抑制 LPS 激活的 RAW264.7 细胞中的 NO 产生（IC ₅₀13.4 ± 0.5 和 12.6 ± 0.4 µM) 与二糖衍生物相比（IC ₅₀范围从 58.9 ± 3.3 到 65.6 ± 3.8 µM）。