This work evaluated the immunomodulatory and anti-infective effects of Cratylia mollis lectin (Cramoll) in a model of wound infection induced by S. aureus. Swiss mice were divided into 3 groups (n = 12/group): non-inoculated (Control group); inoculated with S. aureus (Sa group); inoculated with S. aureus and treated with Cramoll (Sa + Cramoll group). In each animal, one lesion (64 mm²) was induced on the back and contaminated with S. aureus (~4.0 × 10⁶ CFU/wound). The treatment with Cramoll (5 μg/animal/day) started 1-day post-infection (dpi) and extended for 10 days. Clinical parameters (wound size, inflammatory aspects, etc.) were daily recorded; while cytokines levels, bacterial load and histological aspects were determined in the cutaneous tissue at 4^th dpi or 11^th dpi. The mice infected with S. aureus exhibited a delay in wound contraction and the highest inflammatory scores. These effects were impaired by the treatment with Cramoll which reduced the release of key inflammatory mediators (TNF-α, NO, VEGF) and the bacterial load at wound tissue. Histological evaluations showed a restauration of skin structures in the animals treated with Cramoll. Taken together, these results provide more insights about the healing and immunomodulatory properties of Cramoll and suggest this lectin as a lead compound for treatment of wound infection.

这项工作在金黄色葡萄球菌诱导的伤口感染模型中评估了Cratylia mollis凝集素 (Cramoll)的免疫调节和抗感染作用。瑞士小鼠分为3组（n=12 /组）：未接种（对照组）；接种金黄色葡萄球菌（Sa组）；用金黄色葡萄球菌接种并用 Cramoll（Sa + Cramoll 组）处理。在每只动物中，背部有一处损伤（64 mm ²）并被金黄色葡萄球菌污染（~4.0 × 10 ⁶CFU/伤口）。用 Cramoll (5 μg/动物/天) 的治疗在感染后 1 天 (dpi) 开始并延长 10 天。每天记录临床参数（伤口大小、炎症方面等）；^{而在第 4 天或第}11^天时在皮肤组织中测定细胞因子水平、细菌负荷和组织学方面。感染金黄色葡萄球菌的小鼠表现出伤口收缩延迟和炎症评分最高。Cramoll 治疗减少了关键炎症介质（TNF-α、NO、VEGF）的释放和伤口组织的细菌负荷，从而削弱了这些作用。组织学评估显示用 Cramoll 治疗的动物的皮肤结构恢复了。总之，这些结果提供了关于 Cramoll 的愈合和免疫调节特性的更多见解，并表明这种凝集素是治疗伤口感染的先导化合物。