ABSTRACT

Introduction: Magnetic Resonance Imaging (MRI) is a non-invasive imaging modality. However, the effects of MRI on the immune system in the in vivo conditions are yet to be clarified. In this study we explored the effects of routine brain MRI on the protein and mRNA peripheral blood levels of interleukin-6 (IL-6), IL-10, IL-17A and transforming growth factor-beta (TGF-β).

Material and methods: 40 subjects, who referred for brain MRI, were entered for evaluating effects of routine brain MRI on the protein and mRNA peripheral blood levels of IL-6, IL-10, IL-17A and TGF-β. Accordingly, peripheral blood were collected before and 3 hours after MRI from the participants. Protein levels of the cytokines were evaluated using ELISA. Also, mRNA levels were analyzed using Real-Time PCR techniques.

Results: Brain MRI without contrast led to an increase in protein levels of IL-6 in the peripheral serum, but did not change protein and mRNA levels of IL-10, IL-17A and TGF-β. IL-6 mRNA levels after MRI were higher in the participants with mild anxiety compared to those without anxiety.

Conclusion: brain MRI without contrast can induce secretion of IL-6 and may be associated with its functions, such as development of plasma cells or induction of inflammation.

摘要

简介： 磁共振成像 (MRI) 是一种非侵入性成像方式。然而，MRI在体内条件下对免疫系统的影响尚待阐明。在本研究中，我们探讨了常规脑部 MRI 对白细胞介素 6 (IL-6)、IL-10、IL-17A 和转化生长因子-β (TGF-β) 的蛋白质和 mRNA 外周血水平的影响。

材料与方法：40名转诊脑MRI的受试者被纳入评估常规脑MRI对外周血IL-6、IL-10、IL-17A和TGF-β蛋白和mRNA水平的影响。因此，在 MRI 之前和之后 3 小时收集参与者的外周血。使用ELISA评估细胞因子的蛋白质水平。此外，使用实时 PCR 技术分析了 mRNA 水平。

结果: 无造影剂的脑 MRI 导致外周血清中 IL-6 的蛋白质水平增加，但没有改变 IL-10、IL-17A 和 TGF-β 的蛋白质和 mRNA 水平。与没有焦虑的参与者相比，轻度焦虑的参与者在 MRI 后的 IL-6 mRNA 水平更高。

结论：无造影剂的脑部MRI可诱导IL-6的分泌，并可能与其功能有关，如浆细胞的发育或炎症的诱导。