The phenylalanine ammonia lyase genes play crucial role in plant response to biotic and abiotic stresses. In this study, we characterized the role of PAL genes in increasing resistance to the Cassava brown streak virus that causes the economically important cassava brown streak disease (CBSD) on cassava in Africa. The whole transcriptomes of eight cassava varieties differing in resistance to CBSD were obtained at 1, 5 and 8 weeks after CBSV infection. Analysis of RNA-Seq data identified the overexpression of PAL1, PAL2, cinnamic acid and two chalcone synthase genes in CBSD-resistant cassava varieties, which was subsequently confirmed by RT-qPCR. The exogenous application of Acibenzolar-S-Methyl induced PAL1 gene expression to enhance resistance in the susceptible var. Kalawe. In contrast, the silencing of PAL1 by RNA interference led to increased susceptibility of the resistant var. Kaleso to CBSD. PAL1 gene of the phenylpropanoid pathway has a major role in inducing resistance to CBSD in cassava plants and its early induction is key for CBSD resistance.

中文翻译：

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苯丙素途径的 PAL1 基因增加了对木薯中木薯褐条病毒的抗性

苯丙氨酸解氨酶基因在植物对生物和非生物胁迫的反应中起着至关重要的作用。在这项研究中，我们描述了 PAL 基因在提高对木薯褐条病毒的抗性中的作用，这种病毒在非洲木薯上引起了经济上重要的木薯褐条病 (CBSD)。在 CBSV 感染后 1、5 和 8 周获得了 8 个对 CBSD 具有不同抗性的木薯品种的全转录组。RNA-Seq 数据分析确定了 PAL1、PAL2、肉桂酸和两种查尔酮合酶基因在 CBSD 抗性木薯品种中的过表达，随后通过 RT-qPCR 证实了这一点。Acibenzolar-S-Methyl的外源应用诱导PAL1基因表达以增强易感变种的抗性。卡拉维。相比之下，RNA 干扰对 PAL1 的沉默导致抗性 var 的易感性增加。Kaleso 到 CBSD。苯丙素途径的 PAL1 基因在木薯植物对 CBSD 抗性中起主要作用，其早期诱导是 CBSD 抗性的关键。