Although oocyte quality is the dominant factor determining embryo quality, few studies have been conducted to evaluate embryo quality based on the metabolites related to the oocyte. With quantification of the follicular fluid (FF) metabolites, in assisted reproductive technology (ART), this study sought to evaluate the embryo or oocyte quality through an informative approach. An evaluation model consisting of 17 features was generated to distinguish the embryo quality on day 3 post-fertilization, and phosphatidylcholines (PCs) were the key contributors to the evaluation. The model was extended to the patients under different ages and hyperstimulations, and the features were further enriched to facilitate the evaluation of the embryo quality. The metabolites were clustered through pathway analysis, leading to a hypothesis that accumulation of arachidonic acid induced by PCs might weaken embryo quality on day 3 post-fertilization. A discriminating model with metabolic features elicited from follicular fluid was established, which enabled the evaluation of the embryo or oocyte quality even under certain clinical conditions, and the increase of PCs in follicular fluid implies the attenuation of embryo quality on day 3 post-fertilization.

中文翻译：

---

卵泡液中磷脂酰胆碱的增加意味着受精后第 3 天胚胎质量下降

尽管卵母细胞质量是决定胚胎质量的主要因素，但很少有研究根据与卵母细胞相关的代谢物来评估胚胎质量。通过在辅助生殖技术 (ART) 中对卵泡液 (FF) 代谢物进行量化，本研究试图通过一种信息丰富的方法来评估胚胎或卵母细胞的质量。生成了由 17 个特征组成的评估模型，以区分受精后第 3 天的胚胎质量，而磷脂酰胆碱 (PC) 是评估的关键因素。将该模型扩展到不同年龄和过度刺激下的患者，进一步丰富了特征，以方便对胚胎质量的评估。通过通路分析对代谢物进行聚类，导致一种假设，即 PC 诱导的花生四烯酸的积累可能会削弱受精后第 3 天的胚胎质量。建立了具有从卵泡液中引出的代谢特征的判别模型，即使在某些临床条件下也能评估胚胎或卵母细胞的质量，卵泡液中 PCs 的增加意味着受精后第 3 天胚胎质量的衰减。