A coumarin-based biosensor for human hepatocellular carcinoma diagnosis with enhanced brightness and water solubility,Materials Chemistry Frontiers

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A coumarin-based biosensor for human hepatocellular carcinoma diagnosis with enhanced brightness and water solubility
Materials Chemistry Frontiers ( IF 6.0 ) Pub Date : 2021-08-27 , DOI: 10.1039/d1qm00972a
Junyan Ma _{1,

2} , Yaoyu Xu ₃ , Xiangtao Kong ₁ , Yuying Wei ₁ , Dan Meng ₄ , Zhenxing Zhang ₄

Affiliation

Hepatocellular carcinoma (HCC) is one of the most frequent cancers worldwide, which usually presents at a late stage and has a poor prognosis. Thus, there is an urgent need for the development of an effective and reliable imaging method for detection and surgical navigation. Most modern imaging technologies, such as super-resolution microscopy and single-molecule localization microscopy, utilize traditional dyes, which frequently have poor water solubility, biocompatibility, and brightness. In this work, we aimed to improve the water solubility as well as brightness of coumarin-based fluorophores for detecting biothiols by modifying their donor region. Our results demonstrated that 4-piperidinopiperidine as a donor improved the brightness and water solubility greatly. Next, we applied this probe in living cell imaging. We assumed that this probe could be used to detect an abnormal level of glutathione within hepatocellular carcinoma and tested this belief with human hepatocellular carcinoma, which demonstrated that this probe is a powerful chemical tool that could distinguish tumor tissues from normal tissues. To conclude, the probe we constructed has the potential to serve as a novel tool for the clinical diagnosis of hepatocellular carcinoma.

中文翻译：

一种用于人肝细胞癌诊断的基于香豆素的生物传感器，具有增强的亮度和水溶性

肝细胞癌（HCC）是世界范围内最常见的癌症之一，通常出现晚期且预后不良。因此，迫切需要开发一种用于检测和手术导航的有效且可靠的成像方法。大多数现代成像技术，如超分辨率显微镜和单分子定位显微镜，都使用传统染料，这些染料的水溶性、生物相容性和亮度往往较差。在这项工作中，我们旨在通过修饰它们的供体区域来提高用于检测生物硫醇的基于香豆素的荧光团的水溶性和亮度。我们的结果表明，4-哌啶基哌啶作为供体大大提高了亮度和水溶性。接下来，我们将该探针应用于活细胞成像。我们假设该探针可用于检测肝细胞癌中异常水平的谷胱甘肽，并用人类肝细胞癌测试了这一观点，这表明该探针是一种强大的化学工具，可以区分肿瘤组织和正常组织。总之，我们构建的探针有可能作为肝细胞癌临床诊断的新工具。

更新日期：2021-09-09

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