Brazilian Journal of Microbiology ( IF 2.1 ) Pub Date : 2021-09-08 , DOI: 10.1007/s42770-021-00603-2 Soo-Yeon Yang 1 , Seung A Chae 1 , Won Yeong Bang 1 , Minjee Lee 1 , O-Hyun Ban 1, 2 , Jungwoo Yang 1 , Young Hoon Jung 2 , Soo-Jung Kim 3
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This study investigated the anti-inflammatory activity of Lactiplantibacillus plantarum IDCC 3501 isolated from kimchi (Korean fermented food) and its safety. When lipopolysaccharide (LPS)-induced RAW 264.7 macrophages were treated with cell-free supernatant from L. plantarum IDCC 3501, the mRNA expression level of inflammatory markers (i.e., TNF-α, IL-1β, and IL-6) was significantly reduced. In addition, the decreased cell viability by LPS was recovered and NO production in LPS-induced cell was also decreased. For the safety assessment, the genes responsible for antibiotic resistance and virulence were not detected from the genome analysis of this strain. Consistent with this, minimal inhibitory concentrations against various antibiotics, biogenic amines, and d-lactate production, as well as enzymatic and hemolysis activities, indicated that L. plantarum IDCC 3501 did not produce any harmful compounds during fermentation. Furthermore, no acute toxicity and mortality were observed in a murine mouse model. Based on our findings, L. plantarum IDCC 3501 is safe and beneficial for human consumption.
Graphical abstract
中文翻译：
植物乳杆菌 IDCC 3501 的抗炎潜力及其安全性评价
本研究调查了从泡菜（韩国发酵食品）中分离的植物乳杆菌IDCC 3501的抗炎活性及其安全性。当脂多糖 (LPS) 诱导的 RAW 264.7 巨噬细胞用来自植物乳杆菌IDCC 3501的无细胞上清液处理时，炎症标志物（即 TNF-α、IL-1β 和 IL-6）的 mRNA 表达水平显着降低. 此外，LPS 降低的细胞活力得到恢复，LPS 诱导的细胞中 NO 的产生也减少了。对于安全性评估，从该菌株的基因组分析中未检测到与抗生素抗性和毒力有关的基因。与此一致，对各种抗生素、生物胺和d 的最小抑制浓度-乳酸产生以及酶促和溶血活性表明植物乳杆菌IDCC 3501 在发酵过程中不会产生任何有害化合物。此外，在小鼠小鼠模型中未观察到急性毒性和死亡率。根据我们的研究结果，植物乳杆菌IDCC 3501 对人类食用是安全且有益的。
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