The peptide WEKPPVSH from walnut protein hydrolyzate was used to evaluate the antioxidant and anti-inflammatory protective effect on lipopolysaccharide (LPS)-activated BV-2 microglia and its possible mechanism. The results indicated that WEKPPVSH significantly decreased nitric oxide (NO) and reactive oxygen species (ROS) generation in a dose-dependent manner, and significantly up-regulated superoxide dismutase and catalase activities (P < 0.01). Results of enzyme-linked immunosorbent assay (ELISA) showed that WEKPPVSH significantly mitigated the secretion of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) (P < 0.01). Immunofluorescence analysis exhibited that WEKPPVSH down-regulated p65 translocation to the cell nucleus. Western blotting showed that WEKPPVSH up-regulated the expression of nuclear factor erythroid 2-related factor (Nrf2) and heme oxygenase-1 (HO-1), and down-regulated the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), p-IκB/IκB, p-p65/p65 and p-p38/p38. In summary, WEKPPVSH might protect against oxidative stress and inflammation in LPS-stimulated BV-2 microglia by enhancing the Nrf2/HO-1 signaling pathway and blocking the nuclear factor-κB/p38 mitogen – activated protein kinase (NF-κB/p38 MAPK) signaling pathway. The results provided an experimental basis for the research and development of walnut peptide products.

核桃蛋白水解物肽WEKPPVSH用于评估脂多糖（LPS）激活的BV-2小胶质细胞的抗氧化和抗炎保护作用及其可能的机制。结果表明，WEKPPVSH以剂量依赖性方式显着降低一氧化氮（NO）和活性氧（ROS）的产生，并显着上调超氧化物歧化酶和过氧化氢酶活性（P  <0.01）。酶联免疫吸附试验（ELISA）结果显示，WEKPPVSH显着降低肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）和白介素-6（IL-6）的分泌（P < 0.01)。免疫荧光分析表明，WEKPPVSH 下调 p65 向细胞核的易位。Western印迹显示WEKPPVSH上调核因子红细胞2相关因子（Nrf2）和血红素加氧酶-1（HO-1）的表达，下调诱导型一氧化氮合酶（iNOS）、环氧合酶-2的表达(COX-2)、p-IκB/IκB、p-p65/p65 和 p-p38/p38。总之，WEKPPVSH 可能通过增强 Nrf2/HO-1 信号通路和阻断核因子-κB/p38 丝裂原活化蛋白激酶 (NF-κB/p38 MAPK) 来防止 LPS 刺激的 BV-2 小胶质细胞的氧化应激和炎症。 ) 信号通路。研究结果为核桃肽产品的研发提供了实验依据。