Previous work stated that Khaya senegalensis, Anacardium ouest L., Pterocarpus erinaceus, Diospyros mespiliformis, Ocimum gratissimum, Manihot esculenta, Vernonia amygdalina Delile, and Daniellia oliveri have a great potential for the fight against infectious diarrhea. However, data on their antibacterial activity on strains of bacteria responsible for infectious diarrhea are not available. This study is aimed at elucidating the mechanism of action of the antibacterial effect of these plants on some bacterial strains responsible for diarrheal infections. The design of the study included first evaluating the degree of sensitivity of Salmonella typhimurium 14028, Escherichia coli ATCC 25922, Shigella spp., and Salmonella spp. strains to aqueous and hydroethanolic extracts of each plant, followed by the determination of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibiotic power (Pa). This screening was completed with the evaluation of the possible mode of action of the extracts by testing the membrane permeability of these bacterial strains. The data collected indicate that the bacterial strains tested were sensitive to the extracts to varying degrees, except Cassia sieberiana DC and Pseudocedrela kotschyi extracts. For the active extracts, inhibition diameters ranged from 18.33 mm to 7 mm. With the exception of Escherichia coli, all strains were sensitive to the aqueous and hydroethanolic extracts of Anacardium occidentale. MICs vary between 3.37 and 25 mg/ml. Membrane permeability test data show that all active extracts affect the bacterial strains tested by attacking the stability of their outer membrane. For all active extracts, the high percentage of membrane destabilization of the bacteria is significantly () better than that of cefixime used as a reference. Thus, it appears that these extracts can destroy Gram-negative bacteria and increase the fluidity and permeability of their cytoplasmic membrane. The knowledge of the mechanism of action of these extracts is an interesting contribution to the fundamental knowledge on the alternative that medicinal plants represent to antibiotics. These extracts can be used in the management of infectious diarrhea.

中文翻译：

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某些西非药用植物对感染性腹泻相关各种细菌外膜的失稳作用百分比

以前的工作表明，Khaya senegalensis、Anacardium ouest L.、Pterocarpus erinaceus、Diospyros mespiliformis、Ocimum gratissimum、Manihot esculenta、Vernonia amygdalina Delile和Daniellia Oliveri在对抗感染性腹泻方面具有巨大潜力。然而，尚无关于它们对引起感染性腹泻的细菌菌株的抗菌活性的数据。本研究旨在阐明这些植物对某些引起腹泻感染的细菌菌株的抗菌作用的作用机制。该研究的设计包括首先评估鼠伤寒沙门氏菌14028、大肠杆菌ATCC 25922、志贺氏菌属和沙门氏菌的敏感性程度属 菌株对每种植物的水和水乙醇提取物进行分析，然后确定最低抑菌浓度 (MIC)、最低杀菌浓度 (MBC) 和抗生素功效 (Pa)。通过测试这些细菌菌株的膜渗透性来评估提取物的可能作用模式，从而完成了该筛选。收集到的数据表明，除了Cassia sieberiana DC 和Pseudocedrela kotschyi提取物之外，所测试的细菌菌株对提取物的敏感程度不同。对于活性提取物，抑制直径范围为 18.33 毫米至 7 毫米。除大肠杆菌外，所有菌株均对大肠杆菌的水和水乙醇提取物敏感。漆树。MIC 在 3.37 和 25 毫克/毫升之间变化。膜渗透性测试数据表明，所有活性提取物都会通过攻击其外膜的稳定性来影响所测试的细菌菌株。对于所有活性提取物，细菌膜不稳定的高百分比显着（)优于用作参考的头孢克肟。因此，这些提取物似乎可以破坏革兰氏阴性菌并增加其细胞质膜的流动性和渗透性。这些提取物作用机制的知识是对药用植物替代抗生素的基础知识的有趣贡献。这些提取物可用于治疗感染性腹泻。