Hepatitis B virus (HBV) infection is limited through vaccination against HBsAg formulated in the Alum adjuvant. However, this alum-formulated vaccine fails to be preventive in some cases, also known as non-responders. Recent studies have shown the immunomodulatory effect of α-tocopherol in various models. Here, we developed a new formulation for HBsAg using α-tocopherol, followed by assessment of immune responses. Experimental BALB/c mice were immunized with a commercial alum-based vaccine or the one formulated in α-tocopherol at different doses. Mice were immunized subcutaneously with 5 μg of HBsAg with different formulations three times with 2-week intervals. Specific total IgG, IgG1, and IgG2a isotypes of antibodies were measured by ELISA. Immunologic cytokines, such as IFN-γ, IL-4, IL-2, and TNF-α, were also evaluated through commercial ELISA kits. Our results showed that the new α-tocopherol-formulated vaccine had the ability to reinforce specific total IgG responses. Moreover, α-tocopherol in the HBsAg vaccine increased IFN-γ, IL-2, and TNF-α cytokines at higher concentrations; however, the vaccine suppressed IL-4 cytokine release. At a lower concentration of α-tocopherol, the IL-4 cytokine response increased without a positive effect on IFN-γ and TNF-α cytokine response. It seems that α-tocopherol can change the immune responses against HBsAg; however, the type of response depends on the dose of α-tocopherol used in the vaccine formulation.

通过在明矾佐剂中配制的针对 HBsAg 的疫苗接种可以限制乙型肝炎病毒 (HBV) 感染。然而，这种明矾配制的疫苗在某些情况下无法预防，也称为无反应者。最近的研究表明，α-生育酚在各种模型中的免疫调节作用。在这里，我们使用 α-生育酚开发了一种新的 HBsAg 制剂，然后评估了免疫反应。用商业明矾疫苗或在不同剂量的α-生育酚中配制的疫苗对实验性 BALB/c 小鼠进行免疫。用不同配方的 5 μg HBsAg 皮下免疫小鼠 3 次，间隔 2 周。通过ELISA测量抗体的特异性总IgG、IgG1和IgG2a同种型。免疫细胞因子，例如 IFN-γ、IL-4、IL-2 和 TNF-α，还通过商业ELISA试剂盒进行了评估。我们的研究结果表明，新的 α-生育酚配方疫苗具有增强特定总 IgG 反应的能力。此外，HBsAg 疫苗中的α-生育酚在较高浓度下会增加 IFN-γ、IL-2 和 TNF-α 细胞因子；然而，疫苗抑制了 IL-4 细胞因子的释放。在较低浓度的 α-生育酚下，IL-4 细胞因子反应增加，而对 IFN-γ 和 TNF-α 细胞因子反应没有积极影响。似乎α-生育酚可以改变对HBsAg的免疫反应；然而，反应类型取决于疫苗配方中使用的 α-生育酚剂量。和更高浓度的 TNF-α 细胞因子；然而，疫苗抑制了 IL-4 细胞因子的释放。在较低浓度的 α-生育酚下，IL-4 细胞因子反应增加，而对 IFN-γ 和 TNF-α 细胞因子反应没有积极影响。似乎α-生育酚可以改变对HBsAg的免疫反应；然而，反应类型取决于疫苗配方中使用的 α-生育酚剂量。和更高浓度的 TNF-α 细胞因子；然而，疫苗抑制了 IL-4 细胞因子的释放。在较低浓度的 α-生育酚下，IL-4 细胞因子反应增加，而对 IFN-γ 和 TNF-α 细胞因子反应没有积极影响。似乎α-生育酚可以改变对HBsAg的免疫反应；然而，反应类型取决于疫苗配方中使用的 α-生育酚剂量。