Recently the use of Sr²⁺ and Zn²⁺ ions substituted HA as an excellent biomaterial has been reported. To expand the usage to load-bearing applications, like hip replacement, it has been proposed that the material should be used in the form of a coating on the implant surface. In this work, we prepared Sr²⁺ and Zn²⁺ co-substituted HA plasma spray coating at different concentrations on titanium alloy (Ti–6Al–4V). All coatings showed excellent bonding strength with the substrate. The apatite layer formation on the coatings was studied via immersion in SBF. All samples exhibited great capability to induce the apatite layer formation on the coating surface, however, 10Zn5Sr-HA coating inhibited the apatite layer formation. The cell material interaction on the surfaces of the coatings was examined through MC3T3-E1 cell and demonstrated higher biocompatibility, protein adsorption ability, and significantly higher dissolution rate than pure HA coating. However, 10Zn5Sr-HA coating shows cytotoxicity which is confirmed by cell proliferation, cell adhesion, and ALP activity. Besides, some dead cells and poor cellular morphology were also found on the coating surface. The co-substituted HA coating revealed excellent antibacterial ability against bacterial colonization. The addition of Sr²⁺ to the Zn-HA coating effectively decreased the thermal instability, cytotoxicity, and increased performance as compared to HA coating. From the results, it is indicated that the 5Zn10Sr-HA coating has good bonding strength, bioactivity, biocompatibility, and antibacterial properties.

最近已经报道了使用 Sr ²⁺和 Zn ²⁺离子取代 HA 作为优良的生物材料。为了将用途扩展到承重应用，如髋关节置换术，有人建议该材料应以植入物表面涂层的形式使用。在这项工作中，我们制备了 Sr ²⁺和 Zn ²⁺钛合金（Ti-6Al-4V）上不同浓度的共取代HA等离子喷涂涂层。所有涂层都显示出与基材的优异结合强度。通过浸泡在 SBF 中研究了涂层上磷灰石层的形成。所有样品都表现出很强的诱导涂层表面形成磷灰石层的能力，然而，10Zn5Sr-HA涂层抑制了磷灰石层的形成。通过MC3T3-E1细胞检测涂层表面的细胞材料相互作用，表现出比纯HA涂层更高的生物相容性、蛋白质吸附能力和显着更高的溶解速率。然而，10Zn5Sr-HA 涂层显示出细胞毒性，这通过细胞增殖、细胞粘附和 ALP 活性得到证实。此外，涂层表面还发现一些死细胞和较差的细胞形态。共取代的 HA 涂层显示出优异的抗细菌定植抗菌能力。Sr的添加与 HA 涂层相比，Zn-HA 涂层的²⁺有效降低了热不稳定性、细胞毒性和提高的性能。结果表明，5Zn10Sr-HA涂层具有良好的结合强度、生物活性、生物相容性和抗菌性能。