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Spontaneous circulation of active microtubules confined by optical traps
Journal of Biological Physics ( IF 1.8 ) Pub Date : 2021-09-08 , DOI: 10.1007/s10867-021-09578-5
Stephen E Martin 1 , Matthew E Brunner 2 , Joshua M Deutsch 1
Affiliation  

We propose an experiment to demonstrate spontaneous ordering and symmetry breaking of kinesin-driven microtubules confined to an optical trap. Calculations involving the feasibility of such an experiment are first performed which analyze the power needed to confine microtubules and address heating concerns. We then present the results of first-principles simulations of active microtubules confined in such a trap and analyze the types of motion observed by the microtubules as well as the velocity of the surrounding fluid, both near the trap and in the far-field. We find three distinct phases characterized by breaking of distinct symmetries and also analyze the power spectrum of the angular momenta of polymers to further quantify the differences between these phases. Under the correct conditions, microtubules were found to spontaneously align with one another and circle the trap in one direction.



中文翻译:

光阱限制的活性微管的自发循环

我们提出了一个实验来证明限制在光阱中的驱动蛋白驱动的微管的自发排序和对称破坏。首先进行涉及此类实验可行性的计算,分析限制微管和解决加热问题所需的功率。然后,我们展示了限制在这种陷阱中的活动微管的第一性原理模拟结果,并分析了微管观察到的运动类型以及周围流体的速度,无论是在陷阱附近还是在远场。我们发现了三个不同的相,其特征在于不同对称性的破坏,并且还分析了聚合物角动量的功率谱,以进一步量化这些相之间的差异。在正确的条件下,

更新日期:2021-09-09
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