Background

The aim of this study was to elucidate the features of the expression of matrix metalloproteinases inducer—EMMPRIN (EMN) and matrix metalloproteinase 1 (MMP-1) in cell lines and in clinical samples of cervical squamous cell carcinoma (SCC).

Material and methods

The study was carried out using RT-PCR, densitometry and immunohistochemical studies (IHC) on commercial cell lines Siha, Caski, transformed with HPV16; HeLa, and C33A transformed with HPV18, line C33A without HPV, and in clinical samples of SCC and morphologically normal tissue adjacent to the tumor.

Results

The data obtained indicate that the expression of mRNA EMN and MMP-1 occurs in all cell lines at different levels. HPV type and number of genes copies had no effect on expression degree both EMN and MMP-1. Gene expression of EMN and MMP-1 has been investigated in tumor and normal tissues. MMP-1 expression in tumor tissue in SCC, as a rule, has been significantly increased (2–6 times) compared to normal tissue. It was found in 90% of tumor samples. It is known, that MMP-1 promotes the development of invasive and metastatic processes. EMN expression was lower in the tumor tissue than in normal tissue in most cases. An increase in EMN expression was noted only in some cases of SCC.

Conclusion

The data obtained indicate that MMP-1 can serve as a marker of the invasive potential of SCC. EMN, apparently, is not a major factor responsible for MMP-1 expression in SCC. Data are important for understanding the process of tumor development and may have prognostic value for the patient.

中文翻译：

---

宫颈鳞状细胞癌细胞系和临床标本中的间质胶原酶 MMP-1 和 EMMPRIN

背景

本研究旨在阐明基质金属蛋白酶诱导剂EMMPRIN (EMN) 和基质金属蛋白酶1 (MMP-1) 在宫颈鳞状细胞癌(SCC) 细胞系和临床样本中的表达特征。

材料与方法

该研究使用 RT-PCR、光密度测定和免疫组织化学研究 (IHC) 对用 HPV16 转化的商业细胞系 Siha、Caski 进行；HeLa 和 C33A 转化为 HPV18，C33A 系没有 HPV，以及 SCC 和肿瘤附近形态正常组织的临床样本。

结果

获得的数据表明，mRNA EMN和MMP-1的表达在所有细胞系中都以不同的水平出现。HPV类型和基因拷贝数对EMN和MMP-1的表达程度没有影响。已在肿瘤和正常组织中研究了 EMN 和 MMP-1 的基因表达。与正常组织相比，SCC 肿瘤组织中的 MMP-1 表达通常显着增加（2-6 倍）。它存在于 90% 的肿瘤样本中。众所周知，MMP-1 促进侵袭性和转移性过程的发展。在大多数情况下，肿瘤组织中的 EMN 表达低于正常组织。仅在某些 SCC 病例中注意到 EMN 表达的增加。

结论

获得的数据表明MMP-1可以作为SCC侵袭潜力的标志物。显然，EMN 不是导致 SCC 中 MMP-1 表达的主要因素。数据对于了解肿瘤发展的过程很重要，并且可能对患者具有预后价值。