Intron length polymorphism (ILP) markers in genes encoding diketide-CoA synthase (DCS) and curcumin synthase (CURS) showed high identification rates in 13 Curcuma species from Asia. However, the sequences of the intron regions have not yet been analyzed. To elucidate the sequence differences in intron regions of the DCS and CURS genes and to search for specific sequences suitable for the identification of Curcuma species, a large number of sequences were determined through subcloning coupled with sequencing analysis of six Curcuma plant specimens belonging to five species that showed distinct ILP patterns. More than 30 sequences of each region from each specimen were grouped into genes DCS1, DCS2, or CURS1–3 and subsequently the sequences of the same genes were compared. Sequences belonging to the same gene showed inter-species similarity, and thus, these intron sequences were less informative within each single-gene region. The determined sequences from each specimen showed 3–5 kinds of sequence lengths in DCS intron I region, and 5–7 kinds of sequence lengths in CURS intron region. The length of determined sequences and the fragment number in each intron region were different among species, or specimens in C. longa, which were in accordance with the fragment lengths and numbers in their corresponding ILP patterns.

编码二酮化合物辅酶A合酶（DCS）和姜黄素合酶（CURS）的基因中的内含子长度多态性（ILP）标记在来自亚洲的13种姜黄中显示出高识别率。然而，尚未分析内含子区域的序列。为阐明DCS和CURS基因内含子区域的序列差异，寻找适合姜黄属物种鉴定的特异性序列，通过亚克隆结合测序分析，对5个物种的6个姜黄属植物标本进行测序分析，确定了大量序列。显示出不同的 ILP 模式。来自每个标本的每个区域的 30 多个序列被分组为基因DCS1、DCS2或CURS1-3以及随后比较相同基因的序列。属于同一基因的序列显示出种间相似性，因此，这些内含子序列在每个单基因区域内的信息量较少。每个标本的确定序列显示DCS内含子I区有3-5种序列长度，CURS内含子区有5-7种序列长度。确定的序列长度和每个内含子区域的片段数在不同物种或C. longa中的标本之间是不同的，这与其对应的ILP模式中的片段长度和数量一致。