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Multiple haplotypes of Chelonia mydas juveniles in a threatened hotspot area in Southern Brazil.
Genetics and Molecular Biology ( IF 1.7 ) Pub Date : 2021-08-30 , DOI: 10.1590/1678-4685-gmb-2020-0410
Camila Satie Savada 1 , Laura Prosdocimi 2 , Camila Domit 3 , Fernanda Simões de Almeida 1
Affiliation  

Mixed stocks are described for Chelonia mydas and the frequency of haplotypes in feeding areas can aid understanding of the genetic and ecological diversity, since with this information it is possible to identify the origin of the individuals. The current study aims to characterize and compare genetic diversity along the coast of Paraná with 17 other feeding areas in the Atlantic Ocean. A total of 285 samples from juveniles were DNA sequenced in the control region, resulting in the identification of 12 haplotypes, with a predominance of the CMA8 haplotype (69%) and the first registration of CMA23. For the study subjects, haplotypic and nucleotide diversity were 0.469 ± 0.032 and 0.00189 ± 0.00020, respectively, and comparisons with other feeding areas presented significant values for the majority of FST and ΦST. The results point to the importance of this region and provide evidence that over the years a mixed stock has used the region as a feeding area. This variation could be related to sea currents, climatic changes, and oceanographic characteristics that may alter the availability of food, water temperature, and the presence of turtles. The current results can be considered in conservation plans for Chelonia mydas.

中文翻译:

巴西南部受威胁热点地区的海龟幼鱼的多种单倍型。

Chelonia mydas 描述了混合种群,饲养区的单倍型频率有助于了解遗传和生态多样性,因为有了这些信息,就可以确定个体的起源。目前的研究旨在表征和比较巴拉那海岸与大西洋其他 17 个觅食区的遗传多样性。共对来自幼鱼的 285 份样本在对照区域进行 DNA 测序,鉴定出 12 个单倍型,其中 CMA8 单倍型占优势(69%),首次登记为 CMA23。对于研究对象,单倍型和核苷酸多样性分别为 0.469 ± 0.032 和 0.00189 ± 0.00020,并且与其他饲养区的比较显示了大部分 FST 和 ΦST 的显着值。结果表明该地区的重要性,并提供证据表明多年来混合种群将该地区用作饲养区。这种变化可能与海流、气候变化和海洋学特征有关,它们可能会改变食物的供应、水温和海龟的存在。目前的结果可以在 Chelonia mydas 的保护计划中加以考虑。
更新日期:2021-08-30
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