Due to its excellent biocompatibility and anti-inflammatory activity, amniotic membrane (AM) has attracted much attention from scholars. However, its clinical application in vascular reconstruction was limited for poor processability, rapid biodegradation, and insufficient hemocompatibility. A naturally extracted substance with good cytocompatibility, phytic acid (PA), which can quickly form strong and stable hydrogen bonds on the tissue surface, was used to crosslink decellularized AM (DAM) to prepare a novel vascular replacement material. The results showed that PA-fixed AM had excellent mechanical strength and resistance to enzymatic degradation as well as appropriate surface hydrophilicity. Among all samples, 2% PA-fixed specimen showed excellent human umbilical vein endothelial cells (HUVECs)-cytocompatibility and hemocompatibility. It could also stimulate the secretion of vascular endothelial growth factor and endothelin-1 from seeded HUVECs, indicating that PA might promote neovascularization after implantation of PA-fixed specimens. Also, 2% PA-fixed specimen could inhibit the secretion of tumor necrosis factor-α from co-cultured macrophages, thus might reduce the inflammatory response after sample implantation. Finally, the results of ex vivo blood test and in vivo experiments confirmed our deduction that PA might promote neovascularization after implantation. All the results indicated that prepared PA-fixed DAM could be considered as a promising small-diameter vascular replacement material.

羊膜（AM）由于其优良的生物相容性和抗炎活性，引起了学者们的广泛关注。然而，其在血管重建中的临床应用因加工性差、生物降解快、血液相容性不足而受到限制。天然提取的具有良好细胞相容性的物质植酸（PA）可在组织表面快速形成强而稳定的氢键，用于交联脱细胞AM（DAM）制备新型血管替代材料。结果表明，PA固定的AM具有优异的机械强度和抗酶降解性以及适当的表面亲水性。在所有样本中，2% PA 固定标本显示出优异的人脐静脉内皮细胞 (HUVEC) - 细胞相容性和血液相容性。它还可以刺激种子 HUVECs 分泌血管内皮生长因子和 endothelin-1，表明 PA 可能促进 PA 固定标本植入后的新生血管形成。此外，2% PA 固定标本可抑制肿瘤坏死因子的分泌。来自共培养的巨噬细胞的α ，因此可能会减少样品植入后的炎症反应。最后，体外血液测试和体内实验的结果证实了我们的推断，即 PA 可能促进植入后的新生血管形成。所有结果表明，制备的 PA 固定 DAM 可以被认为是一种很有前途的小直径血管替代材料。