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Extended depth of focus ultraviolet imaging compared with laser scanning confocal microscopy for the study of micro-Arthropoda surface texture, with the description of a new species of Brachypodopsis (Acari: Hydrachnidia)
Microscopy Research and Technique ( IF 2.5 ) Pub Date : 2021-09-08 , DOI: 10.1002/jemt.23929
Antonio G Valdecasas 1
Affiliation  

Visualization and representation are two processes at the core of basic biodiversity studies. Visualization involves the examination, sorting, and evaluation of similarities and differences among specimens by specialists who then assign them to the same or different species. It is a cognitive process. Representing involves transmitting the knowledge obtained in the first step to others, usually specialists of the group under study, generally through written descriptions aided by representative drawings and/or images. In this work, I describe a new species of water mite, Brachypodopsis guillermoi n. sp. (Acari, Hydrachnidia), from the island of Coiba off the Pacific coast of Panama, using both laser scanning confocal microscopy and extended depth of focus microscopy with visible (wavelength: 400–700 nm) and ultraviolet (wavelength: 365 nm) light. A comparison of the surface texture representation obtained from these imaging methods suggests that extended depth of focus ultraviolet microscopy can be a cost-effective alternative to laser scanning confocal microscopy for the description of exoskeletal features of micro-arthropods.

中文翻译:

与激光扫描共聚焦显微镜相比,扩展焦深紫外成像用于研究微型节肢动物的表面纹理,并描述了一种新的短足纲(Acari: Hydrachnidia)

可视化和表示是基础生物多样性研究核心的两个过程。可视化涉及由专家检查、分类和评估标本之间的异同,然后将它们分配给相同或不同的物种。这是一个认知过程。代表包括将第一步中获得的知识传递给其他人,通常是研究小组的专家,通常通过有代表性的图纸和/或图像辅助的书面描述。在这项工作中,我描述了一种新的水螨物种Brachypodopsis guillermoin. sp。(Acari, Hydrachnidia),来自巴拿马太平洋沿岸的 Coiba 岛,使用激光扫描共聚焦显微镜和可见光(波长:400-700 nm)和紫外(波长:365 nm)光的扩展焦深显微镜。从这些成像方法获得的表面纹理表示的比较表明,扩展焦深紫外显微镜可以成为激光扫描共聚焦显微镜的一种具有成本效益的替代方法,用于描述微型节肢动物的外骨骼特征。
更新日期:2021-09-08
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