Many analytical challenges in biomedicine arise from the generally high heterogeneity and complexity of glycan- and glycoconjugate-containing samples, which are often only available in minute amounts. Therefore, highly sensitive workflows and detection methods are required. In this review mass spectrometric workflows and detection methods are evaluated for glycans and glycoproteins. Furthermore, glycomic methodologies and innovations that are tailored for enzymatic treatments, chemical derivatization, purification, separation, and detection at high sensitivity are highlighted. The discussion is focused on the analysis of mammalian N-linked and GalNAc-type O-linked glycans.

中文翻译：

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生物医学中的高灵敏度糖组学


生物医学中的许多分析挑战源于含有聚糖和糖复合物的样品通常具有较高的异质性和复杂性，而这些样品通常只能获得微量的样品。因此，需要高度灵敏的工作流程和检测方法。在这篇综述中，评估了聚糖和糖蛋白的质谱工作流程和检测方法。此外，还强调了专为酶处理、化学衍生化、纯化、分离和高灵敏度检测而定制的糖组学方法和创新。讨论的重点是哺乳动物N连接聚糖和 GalNAc 型O连接聚糖的分析。