Promoting cancer cells apoptosis is one of the effective methods to treat cancer. Human hepatocellular carcinoma (HepG2) and colorectal cancer (HCT-116) cell lines were used in the present study to evaluate the cytotoxic and anticancer properties of Nepeta septemcrenata Polysaccharide (NSP). Treatment of the two examined cells with NSP displayed a significant cytotoxicity towards HepG2 in a dose-dependent manner; meanwhile, its effect on HCT-116 was obtained under the influence of low doses. The quantitative real- time PCR (QRT-PCR) investigation revealed that NSP significantly up-regulated the expression levels of p53, p16, Fas, Fas-L, Bax, caspases-3, caspase-9, and TNF-α in association with down-regulation of cyclin D1, TERT, and BCL2. These findings declare the apoptotic characteristic of NSP.NSP, can also inhibit the development of cancer cells through the down-regulation of TGF-β and VEGF. Our results suggested that the polysaccharides isolated from N. septemcrenata possess anticancer properties that could be explored for the development of novel anticancer agents.

中文翻译：

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荆芥多糖细胞毒抗癌作用评价

促进癌细胞凋亡是治疗癌症的有效方法之一。本研究使用人肝细胞癌 (HepG2) 和结直肠癌 (HCT-116) 细胞系来评估荆芥多糖 (NSP) 的细胞毒性和抗癌特性。用 NSP 处理两个检查的细胞显示出对 HepG2 的显着细胞毒性，呈剂量依赖性；同时，其对HCT-116的作用是在低剂量的影响下获得的。实时定量 PCR (QRT-PCR) 研究表明，NSP 显着上调 p53、p16、Fas、Fas-L、Bax、caspases-3、caspase-9 和 TNF-α 的表达水平，与细胞周期蛋白 D1、TERT 和 BCL2 的下调。这些发现表明了 NSP.NSP 的凋亡特征，还可以通过下调 TGF-β 和 VEGF 来抑制癌细胞的发展。我们的结果表明，从 N. septemcrenata 中分离出的多糖具有抗癌特性，可用于开发新型抗癌剂。