This study aimed to investigate the phylogenetic characterization and virulence traits of uropathogenic Escherichia coli (UPEC) isolated from kidney transplant patients (KTPs) as well as non-KTPs and analyze the clonal distribution of Extended spectrum β-lactamases (ESBLs)-producing UPEC containing blaCTX-M gene. To this end, we determined virulence marker and the phylogenetic characterization of UPEC in non-KTPs (n = 65) and KTPs (n = 46). The non-KTPs were considered the control group of the study. Also, according to the Achtman scheme, we performed multilocus sequence typing to assess the relationship between twenty-nine of ESBL-producing isolates containing blaCTX-M gene. According to the results of PCR assay, the prevalence of virulence factor genes ranged from 0% (cnf and papG III) to 93.7% (fimH). Also, KTP isolates significantly differed from non-KTP isolates only in terms of the prevalence of pap GI elements. Moreover, the most frequent UPEC isolates were in phylogenetic group B2, followed by group D (18.9%), and group A (13.5%). Furthermore, except for phylogenetic group C, there was no significant correlation between phylogenetic distribution in KTPs and non-KTPs. Additionally, MLST analysis of blaCTX-M carrying isolates identified 18 unique sequence types (ST) the most common of which was ST131 (24.1%), followed by ST1193 (10.3%), while fourteen STs were detected only once. The results further revealed significant differences between the UPEC isolates from KTPs and non-KTPs regarding the phylogroups C and PAI gene. Based on MLST analysis, we also observed a relatively high diversity in UPEC isolates obtained from KTPs and non-KTPs. Moreover, clonal complex (CC) 131 and ST131 were found to be the most prevalent clones and ST types, respectively. Besides, for the first time, ST8503 were reported in KTPs. These results suggested regular studies on characterization of UPEC isolates among KTPs.

中文翻译：

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伊朗肾移植患者中产生 blaCTX-M 基因的尿路致病性大肠杆菌的分子流行病学：CC131 和 CC10 的克隆传播

本研究旨在调查从肾移植患者 (KTP) 和非 KTP 中分离出的尿路致病性大肠杆菌 (UPEC) 的系统发育特征和毒力特征，并分析产超广谱 β-内酰胺酶 (ESBL) 的 UPEC 的克隆分布。 blaCTX-M 基因。为此，我们确定了非 KTP (n = 65) 和 KTP (n = 46) 中 UPEC 的毒力标记和系统发育特征。非KTPs被认为是研究的对照组。此外，根据 Achtman 方案，我们进行了多位点序列分型，以评估 29 个含有 blaCTX-M 基因的产 ESBL 分离株之间的关系。根据PCR检测结果，毒力因子基因的流行范围为0%（cnf和papG III）至93.7%（fimH）。还，KTP 分离株与非 KTP 分离株的显着差异仅在于 pap GI 元素的流行。此外，最常见的 UPEC 分离株在系统发育组 B2 中，其次是 D 组（18.9%）和 A 组（13.5%）。此外，除了系统发育组C外，KTPs和非KTPs的系统发育分布之间没有显着相关性。此外，对携带 blaCTX-M 的分离株进行的 MLST 分析确定了 18 种独特的序列类型 (ST)，其中最常见的是 ST131 (24.1%)，其次是 ST1193 (10.3%)，而仅检测到 14 种 ST。结果进一步揭示了来自 KTP 和非 KTP 的 UPEC 分离物在系统发育群 C 和 PAI 基因方面的显着差异。基于 MLST 分析，我们还观察到从 KTP 和非 KTP 获得的 UPEC 分离株的多样性相对较高。而且，克隆复合体 (CC) 131 和 ST131 分别被发现是最普遍的克隆和 ST 类型。此外，ST8503 首次出现在 KTP 中。这些结果表明定期研究 KTP 中 UPEC 分离株的特征。