A formulation of black cumin (Nigella sativa L.), licorice (Glycyrrhiza glabra L.), anise (Pimpinella anisum L.) and tea (Camellia sinensis (L.) Kuntze) (denoted BLAB tea) is traditionally used to relief allergy reaction including allergic rhinitis. However, little is known about its underlining mechanism of anti-allergic effects. To investigate the anti-allergenic mechanism of BLAB tea, we treated ovalbumin (OVA)-induced allergic rhinitis (AR) model of mice with BLAB tea, and elucidated its possible mechanism of action. Mice in the control group were treated with phosphate-buffered saline only. Subsequently, the infiltration of different inflammatory cells was measured. In addition, histopathological changes in the nasal mucosa, and the levels of allergen-specific cytokines and OVA-specific immunoglobulins were measured. The aqueous extract of BLAB significantly alleviated the nasal symptoms and reduced the accumulation of inflammatory cells in the nasal mucosa and nasal lavage fluid of AR model of mice. The aqueous extract of BLAB induced the production of Th1 and Treg cytokines and inhibited the release of Th2 cytokines and histamine in nasal mucosa and serum of mice while decreasing the serum levels of OVA-specific IgE, IgG1, and IgG2a. These results suggest the potential of the aqueous extract of BLAB as a treatment option for allergic diseases.

中文翻译：

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黑孜然、甘草、茴香和红茶的提取物通过平衡肺中辅助 T 细胞的活性减轻 OVA 诱导的小鼠过敏性鼻炎

黑孜然 (Nigella sativa L.)、甘草 (Glycyrrhiza glabra L.)、茴香 (Pimpinella anisum L.) 和茶 (Camellia sinensis (L.) Kuntze)（称为 BLAB 茶）的配方传统上用于缓解过敏反应包括过敏性鼻炎。然而，对其抗过敏作用的重要机制知之甚少。为了研究 BLAB 茶的抗过敏机制，我们用 BLAB 茶治疗了卵清蛋白 (OVA) 诱导的过敏性鼻炎 (AR) 小鼠模型，并阐明了其可能的作用机制。对照组的小鼠仅用磷酸盐缓冲盐水处理。随后，测量不同炎症细胞的浸润。此外，还测量了鼻粘膜的组织病理学变化，以及过敏原特异性细胞因子和 OVA 特异性免疫球蛋白的水平。BLAB水提物显着缓解了鼻部症状，减少了AR模型小鼠鼻粘膜和鼻腔灌洗液中炎症细胞的积聚。BLAB水提物诱导Th1和Treg细胞因子的产生，抑制小鼠鼻粘膜和血清中Th2细胞因子和组胺的释放，同时降低血清OVA特异性IgE、IgG1和IgG2a水平。这些结果表明 BLAB 的水提取物作为过敏性疾病的治疗选择的潜力。BLAB水提物诱导Th1和Treg细胞因子的产生，抑制小鼠鼻粘膜和血清中Th2细胞因子和组胺的释放，同时降低血清OVA特异性IgE、IgG1和IgG2a的水平。这些结果表明 BLAB 的水提取物作为过敏性疾病的治疗选择的潜力。BLAB水提物诱导Th1和Treg细胞因子的产生，抑制小鼠鼻粘膜和血清中Th2细胞因子和组胺的释放，同时降低血清OVA特异性IgE、IgG1和IgG2a的水平。这些结果表明 BLAB 的水提取物作为过敏性疾病的治疗选择的潜力。