Journal of Colloid and Interface Science ( IF 9.4 ) Pub Date : 2021-09-08 , DOI: 10.1016/j.jcis.2021.09.037 Eungyeong Park 1 , Sila Jin 1 , Yeonju Park 2 , Shuang Guo 1 , Hyejin Chang 3 , Young Mee Jung 4
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Hypothesis
Due to the intrinsic nature of the surface-enhanced Raman scattering (SERS), the detection of molecules with weak binding affinities toward metal substrates is critical for development of a universal SERS sensing platform. We hypothesized the physical trapping of small pesticide molecules for active hot spot generation using tyramine-mediated crosslinking chemistry and silver nanoparticles (Ag NPs) enhances SERS detection sensitivity.
Experiments
Tyramine-mediated crosslinking chemistry for sensor application was validated by ultraviolet–visible absorption spectroscopy, scanning electron microscopy, dynamic light scattering, and Raman spectroscopy. SERS sensing platform using tyramine-mediated crosslinking reaction was systematically studied for detection of 1,4-dyethylnylbenzene as a model analyte. This sensor system was applied to detect two other pesticides, thiabendazole and 1,2,3,5-tetrachlorobenzene, which have different binding affinities toward metal surfaces.
Findings
The SERS signal of 1,4-dyethylnylbenzene obtained using this sensor system was 3.6 times stronger than that obtained using the Ag colloidal due to the nanogap of approximately 1.3 nm within the generated hot spots. This sensor system based on tyramine-mediated crosslinked Ag NPs was evaluated as a promising tool to achieve a solution based sensitive detection of various pesticide molecules that cannot be adsorbed on the surfaces of typical SERS substrates such as metal nanoparticles.
中文翻译：
使用酪胺药物交联化学将分析物捕获到动态热点中，以设计多功能传感器
假设
由于表面增强拉曼散射 (SERS) 的固有性质，检测对金属基材具有弱结合亲和力的分子对于开发通用 SERS 传感平台至关重要。我们假设使用酪胺介导的交联化学和银纳米粒子 (Ag NPs) 物理捕获小农药分子以产生活性热点，从而提高 SERS 检测灵敏度。
实验
用于传感器应用的酪胺介导的交联化学通过紫外-可见吸收光谱、扫描电子显微镜、动态光散射和拉曼光谱进行了验证。系统地研究了使用酪胺介导的交联反应的 SERS 传感平台，以检测 1,4-二乙基苯作为模型分析物。该传感器系统用于检测其他两种农药，噻菌灵和 1,2,3,5-四氯苯，它们对金属表面具有不同的结合亲和力。
发现
由于生成的热点内的纳米间隙约为 1.3 nm，因此使用该传感器系统获得的 1,4-二乙基苯的 SERS 信号比使用 Ag 胶体获得的信号强 3.6 倍。这种基于酪胺介导的交联银纳米颗粒的传感器系统被评估为一种有前途的工具，可以实现基于溶液的灵敏检测各种农药分子，这些农药分子不能吸附在典型的 SERS 基材（如金属纳米颗粒）的表面上。
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