Taraxacum kok-saghyz Rodin (TKS) is a promising crop that can produce rubber and also contains other active ingredients such as pentacyclic triterpenoid and sterol secondary metabolites. Our aims is to provide a rapid extraction and sensitive ultra-high-performance liquid chromatography tandem mass spectrometric method for the determination of pentacyclic triterpenoids (α-amyrin, β-amyrin, lupeol, and taraxasterol) and sterols (campesterol, stigmasterol, and β-sitosterol) in TKS. We also compared the difference in content of target compounds between TKS and Taraxacum officinale (TO). Response surface methodology (RSM) with Box–Behnken Design (BBD) was employed to optimize liquid-to-material ratio, microwave power, and ultrasonic power to improve the yield of target compounds. Target compounds were separated by a Waters ACQUITY UPLC BEH C₁₈ column and were identified by atmospheric pressure chemical ionization-mass spectrometry. Under optimal conditions (liquid-to-solid ratio of 45 mL/g, microwave power of 420 W, and ultrasonic power of 560 W), the recoveries for target compounds were from 72.63% to 95.94%. The intra/inter-day precision was lower than 5.5%. The limits of detection and quantitation of target compounds were 0.5–5.0 ng/g and 1.0–10.0 ng/g, respectively. The developed method successfully extracted and analyzed the target compounds in TKS. The content of target compounds in TKS (9.44 mg/g) was significantly higher than that in TO (3.35 mg/g). We found that TKS is an excellent species of the Taraxacum genus, which is rich in not only latex rubber but also pentacyclic triterpenes and sterols.

蒲公英（TKS）是一种很有前途的作物，可以生产橡胶，还含有其他活性成分，如五环三萜和甾醇次级代谢产物。我们的目标是提供一种快速提取且灵敏的超高效液相色谱串联质谱法，用于测定五环三萜类化合物（α-香树脂醇、β-香树脂醇、羽扇豆醇和蒲公英甾醇）和甾醇（菜油甾醇、豆甾醇和 β -谷甾醇）在 TKS 中。我们还比较了TKS和蒲公英在目标化合物含量上的差异（到）。采用 Box-Behnken Design (BBD) 的响应面法 (RSM) 来优化液料比、微波功率和超声波功率，以提高目标化合物的产率。目标化合物由 Waters ACQUITY UPLC BEH C _{18 分离}柱，并通过常压化学电离-质谱法进行鉴定。在最佳条件下（液固比45 mL/g，微波功率420 W，超声功率560 W），目标化合物的回收率为72.63%~95.94%。日内/日间精度低于5.5%。目标化合物的检测限和定量限分别为 0.5–5.0 ng/g 和 1.0–10.0 ng/g。开发的方法成功提取并分析了 TKS 中的目标化合物。TKS中目标化合物的含量（9.44 mg/g）明显高于TO（3.35 mg/g）。我们发现，TKS 是蒲公英属的优良品种，它不仅富含乳胶，还富含五环三萜和甾醇。